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Titolo:
Upregulation of ornithine decarboxylase mRNA expression in Barrett's esophagus and Barrett's - Associated adenocarcinoma
Autore:
Brabender, J; Lord, RV; Danenberg, KD; Metzger, R; Schneider, PM; Uetake, H; Kawakami, K; Park, JM; Salonga, D; Peters, JH; DeMeester, TR; Holscher, AH; Danenberg, PV;
Indirizzi:
Univ So Calif, Keck Sch Med, Kenneth Norris Jr Comprehens Canc Ctr, Dept Biochem & Mol Biol, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 ol Biol, Los Angeles, CA 90033 USA Univ So Calif, Keck Sch Med, Dept Surg, Los Angeles, CA 90033 USA Univ So Calif Los Angeles CA USA 90033 pt Surg, Los Angeles, CA 90033 USA Univ Cologne, Dept Visceral & Vasc Surg, Cologne, Germany Univ Cologne Cologne Germany ept Visceral & Vasc Surg, Cologne, Germany
Titolo Testata:
JOURNAL OF GASTROINTESTINAL SURGERY
fascicolo: 2, volume: 5, anno: 2001,
pagine: 174 - 181
SICI:
1091-255X(200103/04)5:2<174:UOODME>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA; POLYAMINE METABOLISM; CELL-TRANSFORMATION; DYSPLASIA; CANCER; GENE; TUMORIGENESIS; PREVALENCE; PCR;
Keywords:
ornithine decarboxylase; ODC; Barrett's esophagus; esophageal neoplasia; esophageal adenocarcinoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Danenberg, PV Univ So Calif, Keck Sch Med, Kenneth Norris Jr Comprehens Canc Ctr, Dept Biochem & Mol Biol, 1303 N Mission Rd,CRL 204, Los Angeles, CA90033 USA Univ So Calif 1303 N Mission Rd,CRL 204 Los Angeles CA USA 90033
Citazione:
J. Brabender et al., "Upregulation of ornithine decarboxylase mRNA expression in Barrett's esophagus and Barrett's - Associated adenocarcinoma", J GASTRO S, 5(2), 2001, pp. 174-181

Abstract

The Barrett's multistage process is characterized histopathologically by progression from Barrett's intestinal metaplasia to Barrett's esophagus withdysplasia and ultimately adenocarcinoma. Understanding the cellular and molecular events in this multistage process may contribute to improved diagnosis and treatment. Ornithine decarboxylase (ODC) is the first enzyme in thebiosynthesis of polyamines. Elevated ODC activity has been found to be associated with progression during Barrett's esophagus, but the regulation of ODC gene expression in the development of Barrett's-associated adenocarcinoma has not been reported. The aim of this study was to assess the prevalence and timing of ODC mRNA expression in the Barrett's metaplasia-dysplasia-adenocarcinoma sequence. ODC mRNA expression levels, relative to the stably expressed internal reference gene p-actin, were measured using a quantitative reverse transcription-polymerase chain reaction (RT-PCR) method (ABI 7700 Sequence Detector System) in 104 specimens from 19 patients with Barrett's esophagus without carcinoma and 22 patients with Barrett's-associated adenocarcinoma. The median ODC mRNA expression levels were significantly increased in Barrett's esophagus tissues compared to matched normal tissues in patients without adenocarcinoma of the esophagus (P = 0.002; Wilcoxon test). A significant progressive increase in ODC mRNA expression was detectable through the stages of the metaplasia-dysplasia-carcinoma sequence in patients with Barrett's-associated adenocarcinoma (r = 0.719; P less than or equalto0.001; Spearman's rho test). These findings show that upregulation of ODC mRNA expression is an early event in the development and progression of Barrett's-associated adenocarcinoma of the esophagus, and they suggest that high ODC mRNA expression levels may be a clinically useful biomarker for the detection of occult adenocarcinoma.

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Documento generato il 05/04/20 alle ore 06:36:20