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Titolo:
Liquid chromatographic determination of ketoconazole, a potent inhibitor of CYP3A4-mediated metabolism
Autore:
de Bruijn, P; Kehrer, DFS; Verweij, J; Sparreboom, A;
Indirizzi:
Rotterdam Canc Inst, Daniel den Hoed Klin, Dept Med Oncol, Lab Expt Chemotherapy & Pharmacol, NL-3008 AE Rotterdam, Netherlands Rotterdam Canc Inst Rotterdam Netherlands NL-3008 AE terdam, Netherlands Univ Rotterdam Hosp, NL-3008 AE Rotterdam, Netherlands Univ Rotterdam Hosp Rotterdam Netherlands NL-3008 AE terdam, Netherlands
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 753, anno: 2001,
pagine: 395 - 400
SICI:
1387-2273(20010405)753:2<395:LCDOKA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLINICAL RELEVANCE; SERUM; PHARMACOKINETICS; BIOASSAY; PLASMA;
Keywords:
ketoconazole;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Sparreboom, A Rotterdam Canc Inst, Dept Med Oncol, Groene Hilledijk 301, NL-3075 EA Rotterdam, Netherlands Rotterdam Canc Inst Groene Hilledijk 301 Rotterdam Netherlands NL-3075 EA
Citazione:
P. de Bruijn et al., "Liquid chromatographic determination of ketoconazole, a potent inhibitor of CYP3A4-mediated metabolism", J CHROMAT B, 753(2), 2001, pp. 395-400

Abstract

A high-performance liquid chromatographic assay with UV detection has beendeveloped for the determination of ketoconazole in human plasma. Quantitative extraction was achieved by a single solvent extraction involving a mixture of acetonitrile-n-butyl chloride (1:4, v/v). Ketoconazole and the internal standard (clotrimazole) were separated on a column packed with InertsilODS-80A material and a mobile phase composed of wa( er-acetonitrile-tetrahydrofuran-ammonium hydroxide-triethylamine (45:50.2:2.5:0.1:0.1, v/v). The column effluent was monitored at a wavelength of 206 nm with a detector range set at 0.5. The calibration graph was linear in the range of 20-2000 ng/ml, with a lower limit of quantitation of 20.0 ng/ml. The extraction recoveries for ketoconazole and clotrimazole in human plasma were 93 +/- 9.7% and83 +/- 10.0%, respectively. The developed method has been successfully applied to a clinical study to examine the pharmacokinetics of ketoconazole ina cancer patient. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 12:01:45