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Titolo:
Internal standard high-performance liquid chromatography method for the determination of obidoxime in urine of organophosphate-poisoned patients
Autore:
Grasshoff, C; Thiermann, H; Gillessen, T; Zilker, T; Szinicz, L;
Indirizzi:
Inst Pharmakol & Toxikol, D-85748 Garching, Germany Inst Pharmakol & Toxikol Garching Germany D-85748 5748 Garching, Germany Tech Univ Munich, Klinikum Rechts Isar, Med Klin 2, Toxikol Abt, D-81664 Munich, Germany Tech Univ Munich Munich Germany D-81664 kol Abt, D-81664 Munich, Germany
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 2, volume: 753, anno: 2001,
pagine: 203 - 208
SICI:
1387-2273(20010405)753:2<203:ISHLCM>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
THERAPY; PHARMACOKINETICS; VALIDATION; TOXOGONIN;
Keywords:
obidoxime; organophosphate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Szinicz, L Inst Pharmakol & Toxikol, Ingolstaedter Landstr 100, D-85748 Garching, Germany Inst Pharmakol & Toxikol Ingolstaedter Landstr 100 Garching Germany D-85748
Citazione:
C. Grasshoff et al., "Internal standard high-performance liquid chromatography method for the determination of obidoxime in urine of organophosphate-poisoned patients", J CHROMAT B, 753(2), 2001, pp. 203-208

Abstract

Obidoxime is an antidote approved for reactivation of inhibited acetylcholinesterase in organophosphate poisoning. HPLC methods were described far its determination in blood or aqueous solutions but not for the determinationin urine. Since data for renal obidoxime excretion ranged from 2.2 to 84% of administered dose in healthy volunteers depending on the route of administration and little is known about pharmacokinetics of obidoxime in severely intoxicated patients we developed an internal standard (HI 6) reversed-phase HPLC method for determining obidoxime in urine. The mobile phase consisted of methanol, the counter ion 1-heptane sulfonic acid and tetrabutylammonium phosphate, the stationary phase involved a 5 mum reversed-phase column(125x4 mm). Obidoxime was detected spectrophotometrically at 288 nm. The limit of quantification (LOQ) was 1 muM, the limit of detection (LOD) 0.5 muM. Linear calibration curves were obtained in a concentration range from 1 to 1000 muM. Intra- and inter-day precision C.V.s were below 4%. Accuracy was 95.9% in the LOQ range. Using this method, we were able to quantify obidoxime in urine of an organophosphate poisoned patient. Based on this data we calculated that 58% of the administered dose was excreted in the urine. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/06/20 alle ore 02:05:11