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Titolo:
Evaluation of immobilized metal affinity chromatography for purification of penicillin acylase
Autore:
Fitton, V; Santarelli, X;
Indirizzi:
Univ Bordeaux 2, UMR 5544, ESTBB, F-33076 Bordeaux, France Univ Bordeaux 2 Bordeaux France F-33076 ESTBB, F-33076 Bordeaux, France
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 754, anno: 2001,
pagine: 135 - 140
SICI:
1387-2273(20010415)754:1<135:EOIMAC>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI ATCC-11105; PROTEINS; BINDING; AMIDASE; MUTANT;
Keywords:
enzymes; penicillin acylase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Santarelli, X Univ Bordeaux 2, UMR 5544, ESTBB, 146 Rue Leo Saignat, F-33076 Bordeaux, France Univ Bordeaux 2 146 Rue Leo Saignat Bordeaux France F-33076
Citazione:
V. Fitton e X. Santarelli, "Evaluation of immobilized metal affinity chromatography for purification of penicillin acylase", J CHROMAT B, 754(1), 2001, pp. 135-140

Abstract

The aim of this work was to test immobilized metal affinity chromatography(TMAC) for the purification of penicillin acylase. After evaluation of different metals, Cu2+ was selected. Different samples were tested: pure penicillin acylase, industrial clarified feedstock and crude extract. After comparing two eluents, NH,CI and imidazole, it appeared that although both gavegood results for recovery and activity, NH,CI was a more selective eluent with a higher fold purification than imidazole (4.64 versus 2.04). Moreover. we shown that a multistep gradient of NH4Cl, greatly increased the degreeof purification (12.36) compared with the one-step process as control (4.64). In addition, good recovery was obtained (97-100%). (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 13:14:26