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Titolo:
Assessment of [F-18]fluoroethylflumazenil metabolites using high-performance liquid chromatography and tandem mass spectrometry
Autore:
Leveque, P; de Hoffmann, E; Labar, D; Gallez, B;
Indirizzi:
Univ Catholique Louvain, Unite Chim Pharmaceut & Radiopharm, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 B-1200 Brussels, Belgium Univ Catholique Louvain, Lab Spectrometrie Masse, B-1348 Louvain, Belgium Univ Catholique Louvain Louvain Belgium B-1348 , B-1348 Louvain, Belgium Univ Catholique Louvain, Unite Tomog Par Positrons, B-1348 Louvain, Belgium Univ Catholique Louvain Louvain Belgium B-1348 , B-1348 Louvain, Belgium Univ Catholique Louvain, Lab Resonance Magnet Biomed, B-1200 Brussels, Belgium Univ Catholique Louvain Brussels Belgium B-1200 B-1200 Brussels, Belgium
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 754, anno: 2001,
pagine: 35 - 44
SICI:
1387-2273(20010415)754:1<35:AO[MUH>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSITRON EMISSION TOMOGRAPHY; CENTRAL BENZODIAZEPINE RECEPTORS; HUMAN BRAIN; BINDING; INVIVO; BABOON;
Keywords:
[F-18]fluoroethylflumazenil; metabolite; mass spectrometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Gallez, B Univ Catholique Louvain, Unite Chim Pharmaceut & Radiopharm, AveMounier 73-40, B-1200 Brussels, Belgium Univ Catholique Louvain Ave Mounier 73-40 Brussels Belgium B-1200
Citazione:
P. Leveque et al., "Assessment of [F-18]fluoroethylflumazenil metabolites using high-performance liquid chromatography and tandem mass spectrometry", J CHROMAT B, 754(1), 2001, pp. 35-44

Abstract

A simple procedure using HPLC and tandem mass spectrometry has been developed for the determination of fluoroethylflumazenil metabolites. Samples were precipitated with acetonitrile, evaporated to dryness followed by reconstitution with methanol. As mobile phase, 50 mM ammonium formate-methanol (58:42, v/v) was used. The method is valid both for cold and radiolabelled metabolites. Various cold metabolites (hydroxylated and/or dealkylated) were identified in rat and human microsome preparations. Radiolabelled metabolites arise from two or more transformations including hydroxylation. The methodology developed can be applied for further characterisation of metabolites, and for the determination of non metabolised [F-18]fluoroethylflumazenil in routine clinical analysis. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 21:24:59