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Titolo:
Determination of the antihypertensive drug cilazapril and its active metabolite cilazaprilat in pharmaceuticals and urine by solid-phase extraction and high-performance liquid chromatography with photometric detection
Autore:
Prieto, JA; Jimenez, RM; Alonso, RM; Ortiz, E;
Indirizzi:
Univ Pais Vasco, Fac Ciencias, Dept Quim Analit, E-48080 Bilbao, Spain Univ Pais Vasco Bilbao Spain E-48080 Quim Analit, E-48080 Bilbao, Spain Univ Pais Vasco, Fac Med & Odontol, Dept Especialidades Med Quirurg, E-48080 Bilbao, Spain Univ Pais Vasco Bilbao Spain E-48080 Med Quirurg, E-48080 Bilbao, Spain
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 754, anno: 2001,
pagine: 23 - 34
SICI:
1387-2273(20010415)754:1<23:DOTADC>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONVERTING ENZYME-INHIBITOR; IONIZATION MASS-SPECTROMETRY; GAS-CHROMATOGRAPHY; QUANTITATIVE-DETERMINATION; AMPEROMETRIC DETECTION; ACE-INHIBITOR; HUMAN-PLASMA; ANGIOTENSIN; CAPTOPRIL; QUINAPRIL;
Keywords:
cilazapril; cilazaprilat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Alonso, RM Univ Pais Vasco, Fac Ciencias, Dept Quim Analit, Apdo 644, E-48080 Bilbao,Spain Univ Pais Vasco Apdo 644 Bilbao Spain E-48080 080 Bilbao,Spain
Citazione:
J.A. Prieto et al., "Determination of the antihypertensive drug cilazapril and its active metabolite cilazaprilat in pharmaceuticals and urine by solid-phase extraction and high-performance liquid chromatography with photometric detection", J CHROMAT B, 754(1), 2001, pp. 23-34

Abstract

A liquid chromatographic method with photometric detection for the determination of cilazapril and its active metabolite and degradation product cilazaprilat in urine and pharmaceuticals has been developed. The chromatographic method consisted of a mu Bondapak C-18 column maintained at 30 +/-0.2 degreesC, using a mixture of methanol-10 mM phosphoric acid (50:50 v/v) as mobile phase at a flow-rate of 1.0 ml/min. Enalapril maleate was used as internal standard. The detection was performed at a wavelength of 206 nm. A study of the retention of cilazapril and cilazaprilat using solid-liquid extraction has been carried out in order to optimise the clean-up procedure for urine samples, which consisted of a solid-liquid extraction using C-8 cartridges. Recoveries greater than 85% are obtained for both compounds. The method was sensitive, precise and accurate enough to be applied to the determination of urine samples obtained from three hypertensive patients up to 24 h after intake of a therapeutic dose (detection limit of 70 ng/ml for cilazapril and cilazaprilat in urine). A comparison of the method developed using photometric and amperometric detection has been carried out. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 14:46:28