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Titolo:
GcpE is involved in the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli
Autore:
Altincicek, B; Kollas, AK; Sanderbrand, S; Wiesner, J; Hintz, M; Beck, E; Jomaa, H;
Indirizzi:
Univ Giessen, Acad Hosp Ctr, Inst Biochem, D-35392 Giessen, Germany Univ Giessen Giessen Germany D-35392 t Biochem, D-35392 Giessen, Germany
Titolo Testata:
JOURNAL OF BACTERIOLOGY
fascicolo: 8, volume: 183, anno: 2001,
pagine: 2411 - 2416
SICI:
0021-9193(200104)183:8<2411:GIIIT2>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE; ISOPENTENYL DIPHOSPHATE BIOSYNTHESIS; MEVALONATE-INDEPENDENT PATHWAY; NONMEVALONATE PATHWAY; TERPENOID BIOSYNTHESIS; 4-(CYTIDINE 5'-DIPHOSPHO)-2-C-METHYL-D-ERYTHRITOL; HIGHER-PLANTS; GENE; SYNTHASE; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Altincicek, B Univ Giessen, Acad Hosp Ctr, Inst Biochem, Friedrichstr 24, D-35392 Giessen, Germany Univ Giessen Friedrichstr 24 Giessen Germany D-35392 ermany
Citazione:
B. Altincicek et al., "GcpE is involved in the 2-C-methyl-D-erythritol 4-phosphate pathway of isoprenoid biosynthesis in Escherichia coli", J BACT, 183(8), 2001, pp. 2411-2416

Abstract

In a variety of organisms, including plants and several eubacteria, isoprenoids are synthesized by the mevalonate-independent 2-C-methyl-D-erythritol4-phosphate (MEP) pathway. Although different enzymes of this pathway havebeen described, the terminal biosynthetic steps of the MEP pathway have not been fully elucidated. In this work, we demonstrate that the gcpE gene ofEscherichia coli is involved in this pathway. E. coli cells were genetically engineered to utilize exogenously provided mevalonate for isoprenoid biosynthesis by the mevalonate pathway. These cells were then deleted for the essential gcpE gene and were viable only if the medium was supplemented with mevalonate or the cells were complemented with an episomal copy of gcpE.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:05:27