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Titolo:
Cross-reactivity studies of a new group 2 allergen from the dust mite Glycyphagus domesticus, Gly d 2, and group 2 allergens from Dermatophagoides pteronyssinus, Lepidoglyphus destructor, and Tyrophagus putrescentiae with recombinant allergens
Autore:
Gafvelin, G; Johansson, E; Lundin, A; Smith, AM; Chapman, MD; Benjamin, DC; Derewenda, U; van Hage-Hamsten, M;
Indirizzi:
Karolinska Hosp & Inst, Dept Med, Unit Clin Immunol & Allergy, Div Clin Immunol & Allergy, S-17176 Stockholm, Sweden Karolinska Hosp & Inst Stockholm Sweden S-17176 -17176 Stockholm, Sweden Univ Virginia, Dept Med, Charlottesville, VA USA Univ Virginia Charlottesville VA USA , Dept Med, Charlottesville, VA USA Univ Virginia, Dept Med & Microbiol, Charlottesville, VA USA Univ Virginia Charlottesville VA USA Microbiol, Charlottesville, VA USA Univ Virginia, Dept Microbiol, Charlottesville, VA USA Univ Virginia Charlottesville VA USA Microbiol, Charlottesville, VA USA Univ Virginia, Dept Mol Physiol, Asthma & Allerg Dis Ctr, Charlottesville,VA USA Univ Virginia Charlottesville VA USA erg Dis Ctr, Charlottesville,VA USA
Titolo Testata:
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
fascicolo: 3, volume: 107, anno: 2001,
pagine: 511 - 518
SICI:
0091-6749(200103)107:3<511:CSOANG>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-BINDING PROTEINS; HOUSE-DUST; STORAGE MITES; ACARUS-SIRO; BLOMIA-TROPICALIS; URBAN-POPULATION; SWEDISH FARMERS; CDNA ANALYSIS; IGE; SENSITIZATION;
Keywords:
cross-reactivity; Der p 2; Glycyphagus domesticus; Gly d 2; Lep d 2; mite; recombinant allergen; Tyr p 2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Gafvelin, G Karolinska Hosp & Inst, Dept Med, Unit Clin Immunol & Allergy,Div Clin Immunol & Allergy, S-17176 Stockholm, Sweden Karolinska Hosp & Inst Stockholm Sweden S-17176 holm, Sweden
Citazione:
G. Gafvelin et al., "Cross-reactivity studies of a new group 2 allergen from the dust mite Glycyphagus domesticus, Gly d 2, and group 2 allergens from Dermatophagoides pteronyssinus, Lepidoglyphus destructor, and Tyrophagus putrescentiae with recombinant allergens", J ALLERG CL, 107(3), 2001, pp. 511-518

Abstract

Background: Dust mites are important inducers of allergic disease. Group 2allergens are recognized as major allergens in several mite species, including Dermatophagoides pteroronyssinus, Lepidoglyphus destructor, and Tyrophagus putrescentiae. No allergens have thus far been characterized on the molecular level from the dust mite Glycyphagus domesticus. Objective: We sought to examine the cross-reactivity among group 2 allergens of G domesticus, L destructor, T putrescentiae, and D pteronyssinus. Methods: A group 2 allergen from G domesticus, Gly d 2, was cloned and expressed as a recombinant protein. Cross-reactivity between Gly d 2 and 3 other group 2 allergens, Lep d 2,Tyr p 2, and Der p 2, was studied by using individual sera and a serum pool RAST-positive to G domesticus, L destructor,T putrescentiae, and D pteronyssinus. Recombinant allergens were used as inhibitors of IgE binding in immunoblotting experiments. Molecular modeling on the basis of the Der p 2 structure was carried out for Gly d 2, Lep d 2,and Tyr p 2. Results: Two cDNAs encoding isoforms of Gly d 2 were isolated, but only the Gly d 2.02 isoform was used in this study Sixteen of 17 subjects had IgE to Gly d 2. The protein sequence of Gly d 2 revealed 79% identity to Lep d 2 and 46% and 41% identity to Tyr p 2 and Der p 2, respectively. Extensive crossreactivity was demonstrated among Gly d 2, Lep d 2, and Tyr p 2, but little cross-reactivity was found between these allergens and Der p 2. According to the tertiary structure of Der p 2 and 3-dimensional models of Gly d2, Lep d 2, and Tyr p 2, differences reside mainly in surface-exposed residues. Conclusion: Gly d 2 showed high sequence homology to Lep d 2. Cross-reactivity was observed between Gly d 2, Lep d 2, and Tyr p 2, but only limited cross-reactivity was demonstrated between these 3 allergens and Der p 2.

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Documento generato il 20/09/20 alle ore 04:40:44