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Titolo:
The relative contribution of mast cell subsets to conjunctival T(H)2-like cytokines
Autore:
Anderson, DF; Zhang, SL; Bradding, P; McGill, JI; Holgate, ST; Roche, WR;
Indirizzi:
Southampton Eye Unit, Southampton SO16 6YD, Hants, England Southampton EyeUnit Southampton Hants England SO16 6YD D, Hants, England Univ Southampton, Dept Pathol, Southampton SO9 5NH, Hants, England Univ Southampton Southampton Hants England SO9 5NH O9 5NH, Hants, England Univ Southampton, Dept Immunopharmacol, Southampton SO9 5NH, Hants, England Univ Southampton Southampton Hants England SO9 5NH O9 5NH, Hants, England
Titolo Testata:
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
fascicolo: 5, volume: 42, anno: 2001,
pagine: 995 - 1001
SICI:
0146-0404(200104)42:5<995:TRCOMC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
FC-EPSILON-RI; ALLERGIC EYE DISEASE; IL-4; KERATOCONJUNCTIVITIS; IMMUNOLOCALIZATION; EXPRESSION; CHALLENGE; NUMBERS; MUCOSA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Anderson, DF Southampton Eye Unit, Tremona Rd, Southampton SO16 6YD, Hants, England Southampton Eye Unit Tremona Rd Southampton Hants England SO16 6YD
Citazione:
D.F. Anderson et al., "The relative contribution of mast cell subsets to conjunctival T(H)2-like cytokines", INV OPHTH V, 42(5), 2001, pp. 995-1001

Abstract

PURPOSE. To investigate the distribution of the T-helper (T-H)2-like cytokines, interleukin (IL)-4, IL-5, IL-G, and IL-13 between mast cell subsets in conjunctival biopsy specimens from normal subjects and those with seasonal allergic conjunctivitis (SAC) during and outside of the grass pollen season. METHODS. Sequential and double in situ hybridization (ISH) and immunohistochemistry (IHC) were performed on thin sections of human conjunctiva to determine the colocalization of the immunoreactivity of IL-4 IL-5, IL-6, and IL-13 to mast cell subsets in normal subjects and subjects with atopy and todetect IL-4 mRNA in conjunctival mast cells. RESULTS. More than 90% of IL-4(+)-immunoreactive cells were observed to bemast cells in conjunctival biopsy specimens from all patient groups. The majority of IL-5(+), IL-6(+), and IL-13(+) cells were also noted to be mast cells for each group. IL-4 preferentially colocalized to the tryptase-chymase(+) mast cell phenotype (MCTC) with MCTC cells comprising 93.3% of cytokine(+) mast cells in symptomatic SAC (P = 0.0017, 89.2% in asymptomatic SAC (P = 0.0008), and 77.8% in normal subjects (P = 0.0472). IL-13 appeared to colocalize preferentially to the MCTC phenotype and IL-5 and IL-6 to the MC, phenotype. ISH showed that 75.8% of mast cells in normal subjects, 78.7%:in subjects with symptomatic SAG, and 18.7% in subjects with asymptomatic SAC expressed mRNA for IL-4. CONCLUSIONS. Conjunctival mast cells are an important source of IL-4, IL-5, IL-6, and IL-13 immunoreactivity. with preferential colocalization of IL-4 and IL-13 on the MCTC subset and IL-5 and IL-6 to the MC, subset. This evidence suggests that differences in protease phenotype may also reflect functional differences evidenced by the different patterns of cytokine distribution.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 10:58:35