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Titolo:
Impaired neurotransmitter release from lacrimal and salivary gland nerves of a murine model of Sjogren's syndrome
Autore:
Zoukhri, D; Kublin, CL;
Indirizzi:
Harvard Univ, Sch Med, Schepens Eye Res Inst, Dept Ophthalmol, Boston, MA 02114 USA Harvard Univ Boston MA USA 02114 t, Dept Ophthalmol, Boston, MA 02114 USA
Titolo Testata:
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
fascicolo: 5, volume: 42, anno: 2001,
pagine: 925 - 932
SICI:
0146-0404(200104)42:5<925:INRFLA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRICHINELLA-INFECTED RATS; MYENTERIC PLEXUS; NOREPINEPHRINE RELEASE; ACETYLCHOLINE-RELEASE; MICE; INNERVATION; SECRETION; AUTOANTIBODIES; INFLAMMATION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Zoukhri, D Harvard Univ, Sch Med, Schepens Eye Res Inst, Dept Ophthalmol, 20 Staniford St, Boston, MA 02114 USA Harvard Univ 20 Staniford St Boston MA USA 02114 , MA 02114 USA
Citazione:
D. Zoukhri e C.L. Kublin, "Impaired neurotransmitter release from lacrimal and salivary gland nerves of a murine model of Sjogren's syndrome", INV OPHTH V, 42(5), 2001, pp. 925-932

Abstract

PURPOSE. To determine whether lacrimal and salivary gland nerves of an animal model of Sjogren's syndrome, the MRL/lpr mouse, are able to release acetylcholine. The second purpose was to determine whether activation of the lacrimal gland nerves of the MRL/lpr mouse leads to protein secretionMETHODS. Total saliva was collected for 10 minutes from the oral cavity ofmale and female MRL/lpr and MRL/+ mice, after intraperitoneal stimulation with pilocarpine and isoproterenol. Lacrimal and salivary gland lobules prepared from 18-week-old MRL/lpr and MRL/+ mice were incubated in the presence of depolarizing KCL (75 mM) solution. Acetylcholine release and peroxidase secretion (a protein secreted by the lacrimal gland) were measured using a spectrofluorometric assay. RESULTS. Female, but not male, MRL/lpr mouse salivary glands were hyper-responsive to in vivo injection of secretagogues. These mice produced significantly higher amounts of saliva than did age-matched MRL/+ mice. Lacrimal and salivary gland nerves from 18-week-old MRL/+ mice released acetylcholinein response to a depolarizing KCI solution. In contrast, nerves in glands from 18-week-old MRL/lpr mice did not increase acetylcholine release in response to the depolarizing solution. Moreover, lacrimal glands from 18-week-old MRL/+ mice were able to secrete peroxidase in response to a depolarizing KCI solution, whereas those from 18-week-old MRL/lpr could not. This was not due to a defect in the secretory process, because addition of an exogenous secretagogue elicited peroxidase secretion from 18-week-old MRL/lpr as well as MRL/+ mice lacrimal glands. CONCLUSIONS. The results show that activation of nerves of lacrimal and salivary glands infiltrated with lymphocytes does not increase the release ofneurotransmitters, which results in impaired secretion from these glands.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:31:28