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Titolo:
Effects of human hydrosalpinx fluid on in-vitro murine fertilization
Autore:
Arrighi, CD; Lucas, H; El-Mowafi, D; Campana, A; Chardonnens, D;
Indirizzi:
Hop Univ Geneva, Dept Obstet & Gynaecol, Clin Steril & Endocrinol Gynecol,CH-1211 Geneva 14, Switzerland Hop Univ Geneva Geneva Switzerland 14 col,CH-1211 Geneva 14, Switzerland
Titolo Testata:
HUMAN REPRODUCTION
fascicolo: 4, volume: 16, anno: 2001,
pagine: 676 - 682
SICI:
0268-1161(200104)16:4<676:EOHHFO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERITONEAL-FLUID; ADVERSELY AFFECT; PREGNANCY RATES; EMBRYO DEVELOPMENT; ADHESION MOLECULES; INTEGRIN SUBUNITS; HUMAN SPERMATOZOA; INFERTILE WOMEN; SPERM MOTILITY; IMPLANTATION;
Keywords:
embryo; hydrosalpinx fluid; IVF; oocyte; spermatozoa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Arrighi, CD Hop Univ Geneva, Dept Obstet & Gynaecol, Clin Steril & Endocrinol Gynecol,30 Bd Cluse, CH-1211 Geneva 14, Switzerland Hop Univ Geneva 30 Bd Cluse Geneva Switzerland 14 Switzerland
Citazione:
C.D. Arrighi et al., "Effects of human hydrosalpinx fluid on in-vitro murine fertilization", HUM REPR, 16(4), 2001, pp. 676-682

Abstract

Patients with hydrosalpinges show a decrease of both fertility and clinical outcome of IVF and embryo transfer treatment. Several reports have demonstrated the negative effects of hydrosalpinx fluid (HSF) on embryo development and implantation. The aim of this study was to determine whether human HSF, collected from infertile patients, might exhibit a deleterious effect on gametes and fertilization using a murine IVF system. Murine gametes were co-incubated during IVF until first cleavage with human HSF diluted to 50% from four patients (HSF1-4). It was demonstrated that HSF affected fertilization, as determined by the count of the 2-cell embryos. Pre-incubation of spermatozoa with HSF during capacitation significantly lowered the percentage of 2-cell embryos (P < 0.05), While HSF1-3 had no significant effect on motility and viability of spermatozoa, HSF4 almost completely affected their survival, In contrast, pre-incubation of ovulated oocytes surrounded by their cumulus cells with HSF before IVF did not impede first cleavage. Takentogether, these results suggest that HSF has a cytotoxic effect on spermatozoa and/or impairs the fertilization process, probably by altering capacitation/acrosome reaction and/or ligand(s)receptor(s) interactions. Hydrosalpinges may be partly associated with sterility through HSF inhibitory effects on fertilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/10/20 alle ore 13:03:39