Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Prevention of hepatitis C virus infection in a chimpanzee by vaccination and epitope mapping of antiserum directed against hypervariable region 1
Autore:
Goto, J; Nishimura, S; Esumi, M; Makizumi, K; Rikihisa, T; Nishihara, T; Mizuno, K; Zhou, YH; Shikata, T; Fujiyama, S; Tomita, K;
Indirizzi:
Kumamoto Univ, Sch Med, Dept Internal Med 3, Kumamoto 8600811, Japan Kumamoto Univ Kumamoto Japan 8600811 rnal Med 3, Kumamoto 8600811, Japan Chemo Sero Therapeut Res Inst, Kumamoto 8691205, Japan Chemo Sero Therapeut Res Inst Kumamoto Japan 8691205 moto 8691205, Japan Nihon Univ, Sch Med, Dept Pathol, Tokyo 1738610, Japan Nihon Univ Tokyo Japan 1738610 ch Med, Dept Pathol, Tokyo 1738610, Japan
Titolo Testata:
HEPATOLOGY RESEARCH
fascicolo: 3, volume: 19, anno: 2001,
pagine: 270 - 283
SICI:
1386-6346(200103)19:3<270:POHCVI>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
NON-A; ANTIBODIES; ENVELOPE; PROTEINS; SEQUENCE; CELLS; HCV; GLYCOPROTEINS; IMMUNITY; VARIANTS;
Keywords:
hepatitis C virus; hypervariable region 1; vaccination; epitope mapping;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Goto, J Kumamoto Univ, Sch Med, Dept Internal Med 3, Kumamoto 8600811, Japan Kumamoto Univ Kumamoto Japan 8600811 3, Kumamoto 8600811, Japan
Citazione:
J. Goto et al., "Prevention of hepatitis C virus infection in a chimpanzee by vaccination and epitope mapping of antiserum directed against hypervariable region 1", HEPATOL RES, 19(3), 2001, pp. 270-283

Abstract

We previously reported on a chimpanzee immunized with both putative envelope glycoproteins (E1 and E2) of hepatitis C virus (HCV), strain HCV-N2, andsynthetic peptides of hypervariable region 1 (HVR1) of a different isolate, HCV-#6. The chimpanzee suggesting that an immune response to the HVR1 is more essential in protecting protection against HCV-#6 infection only when the titer of anti-HVR1 increased, chimpanzee from HCV infection than an immune response to E1 and E2. In this study, immunized this chimpanzee with only synthetic HVR1 peptides after anti-E1 and antibody levels dropped and then rechallenged with 10 infectious chimpanzee doses ai The immunized animalwas protected, and neutralization of HCV with the antiserum the protected animal was achieved by inoculating another chimpanzee with HCV preneutralized by this antiserum mixture. Epitope analysis of HVR1 by Pin-ELISA using antiserum seemed to demonstrate that the antibody response was directed mainly against the C terminus of HVR1. Moreover, our results showed that, if a part of the sequences was conserved, a broad cross-reactivity of the antiserum could be observed, even if amino-acid sequences in this epitope were substituted for those of other HCV strains. (C) 2001 Elsevier Science IrelandLtd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 18:31:22