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Titolo:
Sustained expression of naked plasmid DNA encoding hepatocyte growth factor in mice promotes liver and overall body growth
Autore:
Yang, JW; Chen, SP; Huang, L; Michalopoulos, GK; Liu, YH;
Indirizzi:
Univ Pittsburgh, Sch Med, Dept Pathol, Pittsburgh, PA 15261 USA Univ Pittsburgh Pittsburgh PA USA 15261 Pathol, Pittsburgh, PA 15261 USA Peking Union Med Coll, Dept Cell Biol, Beijing, Peoples R China Peking Union Med Coll Beijing Peoples R China Beijing, Peoples R China Univ Pittsburgh, Sch Pharm, Ctr Pharmacogenet, Pittsburgh, PA USA Univ Pittsburgh Pittsburgh PA USA Ctr Pharmacogenet, Pittsburgh, PA USA
Titolo Testata:
HEPATOLOGY
fascicolo: 4, volume: 33, anno: 2001,
pagine: 848 - 859
SICI:
0270-9139(200104)33:4<848:SEONPD>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE-RENAL-FAILURE; ADULT-RAT LIVER; TRANSGENIC MICE; GENE-THERAPY; FIBROSIS; INJURY; REGENERATION; DISEASE; TRANSFECTION; MECHANISMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Liu, YH Univ Pittsburgh, Sch Med, Dept Pathol, S-405 Biomed Sci Tower,200 Lothrop St, Pittsburgh, PA 15261 USA Univ Pittsburgh S-405 Biomed Sci Tower,200 Lothrop St Pittsburgh PA USA 15261
Citazione:
J.W. Yang et al., "Sustained expression of naked plasmid DNA encoding hepatocyte growth factor in mice promotes liver and overall body growth", HEPATOLOGY, 33(4), 2001, pp. 848-859

Abstract

To understand the physiological functions of exogenous hepatocyte growth factor (HGF) on normal adult animals, we delivered human HGF gene into mice by a hydrodynamics-based in vivo gene transfection approach using a naked plasmid vector. Systemic administration of naked plasmid containing HGF cDNAdriven under cytomegalovirus promoter (pCMV-HGF) by rapid injection via the tail vein produced a remarkable level of human HGF protein in the circulation, beginning to appear at 4 hours and peaking at 12 hours following injection. Tissue distribution studies identified the liver as the organ with the highest level of transgene expression. Through weekly repeated injections of plasmid vector, we achieved sustained, long-term, high levels of exogenous HGF expression in mice for 8 weeks. Increases of more than 31% and 16%in liver and body weights were found, respectively, in the mice that received pCMV-HGF plasmid compared with that given the control vector for 8 weeks. Expression of exogenous HGF in vivo activated mitogen-activated protein kinases and induced proliferating cell nuclear antigen expression in normaladult liver and kidneys. These data suggest that systemic administration of naked plasmid vector is a convenient, safe, and highly efficient approachto introduce and maintain exogenous HGF gene expression in vivo in a controllable fashion. Our results also indicate that long-term expression of human HGF in mice markedly activates growth-related signal transduction events, promotes cell proliferation, and leads to liver and overall body growth in whole adult animals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 16:04:19