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Titolo:
Construction of a genetic map of barley (Hordeum vulgare L.) cross 'Azumamugi' x 'Kanto Nakate Gold' using a simple and efficient amplified fragment-length polymorphism system
Autore:
Mano, Y; Kawasaki, S; Takaiwa, F; Komatsuda, T;
Indirizzi:
Natl Inst Agrobiol Resources, Tsukuba, Ibaraki 3058602, Japan Natl Inst Agrobiol Resources Tsukuba Ibaraki Japan 3058602 3058602, Japan
Titolo Testata:
GENOME
fascicolo: 2, volume: 44, anno: 2001,
pagine: 284 - 292
SICI:
0831-2796(200104)44:2<284:COAGMO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
AFLP MARKERS; SHOOT DIFFERENTIATION; LOCUS; RFLP; PRODUCTS; GENOME;
Keywords:
amplified fragment length polymorphism (AFLP); Hordeum vulgare; linkage map; recombinant inbred line (RIL); sequence-tagged site (STS);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Mano, Y Natl Grassland Res Inst, Nishinasuno, Tochigi 3292793, Japan Natl Grassland Res Inst Nishinasuno Tochigi Japan 3292793 , Japan
Citazione:
Y. Mano et al., "Construction of a genetic map of barley (Hordeum vulgare L.) cross 'Azumamugi' x 'Kanto Nakate Gold' using a simple and efficient amplified fragment-length polymorphism system", GENOME, 44(2), 2001, pp. 284-292

Abstract

We have devised a simple and efficient amplified fragment-length polymorphism (AFLP) system consisting of small slab gels, a discontinuous buffer system, and silver staining. Using this system, a single worker developed a barley map with 227 polymorphic fragments in 2 months. As a mapping population, 99 recombinant inbred lines of barley cultivars 'Azumamugi' x 'Kanto Nakate Gold' were used. Most of the 227 AFLP fragments showed a Mendelian segregation ratio of 1:1, and all were assigned to the seven barley chromosomes. Thus, these fragments are useful as molecular markers. They were integrated with 40 previously characterized sequence-tagged sites, 3 isozymes, and 2 morphological markers to construct an integrated map. The resulting map covered 925.6 cM with 272 markers (detecting 150 loci) at an average interval of 6.5 cM/locus. This system greatly simplifies map construction.

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Documento generato il 15/07/20 alle ore 08:19:59