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Titolo:
Melon bacterial artificial chromosome (BAC) library construction using improved methods and identification of clones linked to the locus conferring resistance to melon Fusarium wilt (Fom-2)
Autore:
Luo, MZ; Wang, YH; Frisch, D; Joobeur, T; Wing, RA; Dean, RA;
Indirizzi:
Clemson Univ, Inst Genom, Clemson, SC 29634 USA Clemson Univ Clemson SC USA 29634 Univ, Inst Genom, Clemson, SC 29634 USA N Carolina State Univ, Dept Plant Pathol, Fungal Genom Lab, Raleigh, NC 27606 USA N Carolina State Univ Raleigh NC USA 27606 nom Lab, Raleigh, NC 27606 USA
Titolo Testata:
GENOME
fascicolo: 2, volume: 44, anno: 2001,
pagine: 154 - 162
SICI:
0831-2796(200104)44:2<154:MBAC(L>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYST-NEMATODE RESISTANCE; MAP-BASED CLONING; DISEASE RESISTANCE; ESCHERICHIA-COLI; DIVERGENT SELECTION; STABLE MAINTENANCE; DNA FRAGMENTS; GENE; TOMATO; SEQUENCE;
Keywords:
bacterial artificial chromosome (BAC) library; Fusarium wilt; melon; pCUGIBAC1; resistant gene;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Luo, MZ Clemson Univ, Inst Genom, 100 Jordan Hall, Clemson, SC 29634 USA Clemson Univ 100 Jordan Hall Clemson SC USA 29634 on, SC 29634 USA
Citazione:
M.Z. Luo et al., "Melon bacterial artificial chromosome (BAC) library construction using improved methods and identification of clones linked to the locus conferring resistance to melon Fusarium wilt (Fom-2)", GENOME, 44(2), 2001, pp. 154-162

Abstract

Utilizing improved methods, two bacterial artificial chromosome (BAC) libraries were constructed for the multidisease-resistant line of melon MR-1. The HindIII library consists of 177 microtiter plates in a 384-well format, while the EcoRI library consists of 222 microtiter plates. Approximately 95.6% of the HindIII library clones contain nuclear DNA inserts with an average size of 118 kb, providing a coverage of 15.4 genome equivalents. Similarly, 96% of the EcoRI library clones contain nuclear DNA inserts with an average size of 114 kb, providing a coverage of 18.7 genome equivalents. Both libraries were evaluated for contamination with high-copy vector, empty pIndigoBac536 vector, and organellar DNA sequences. High-density filters were screened with two genetic markers FM and AM that co-segregate with Fom-2, agene conferring resistance to races 0 and 1 of Fusarium wilt. Fourteen and18 candidate BAC clones were identified for the FM and AM probes, respectively, from the HindIII library, while 34 were identified for the AM probe from filters A, B, and C of the EcoRI library.

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Documento generato il 27/09/20 alle ore 04:44:00