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Titolo:
Effects of reactive oxygen species on aspects of excitation-contraction coupling in chemically skinned rabbit diaphragm muscle fibres
Autore:
Darnley, GM; Duke, AM; Steele, DS; MacFarlane, NG;
Indirizzi:
Univ Glasgow, Ctr Exercise Sci & Med, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland Univ Glasgow Glasgow Lanark Scotland G12 8QQ ow G12 8QQ, Lanark, Scotland Univ Leeds, Sch Biol, Leeds LS2 9JT, W Yorkshire, England Univ Leeds Leeds W Yorkshire England LS2 9JT S2 9JT, W Yorkshire, England
Titolo Testata:
EXPERIMENTAL PHYSIOLOGY
fascicolo: 2, volume: 86, anno: 2001,
pagine: 161 - 168
SICI:
0958-0670(200103)86:2<161:EOROSO>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
SARCOPLASMIC-RETICULUM; SKELETAL-MUSCLE; FREE-RADICALS; RAT; TWITCH; FIBERS; TRABECULAE; STRENGTH; RELEASE; FATIGUE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Darnley, GM Univ Glasgow, Ctr Exercise Sci & Med, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland Univ Glasgow Glasgow Lanark Scotland G128QQ Lanark, Scotland
Citazione:
G.M. Darnley et al., "Effects of reactive oxygen species on aspects of excitation-contraction coupling in chemically skinned rabbit diaphragm muscle fibres", EXP PHYSIOL, 86(2), 2001, pp. 161-168

Abstract

Oxidants have been suggested to enhance contractile function in unfatiguedmuscle. In this study we aimed to determine the effect of oxidants on 'chemically skinned' diaphragm muscle fibre bundles. The sarcoplasmic reticulumand contractile proteins were exposed to superoxide anions (O-2(-)) and hydrogen peroxide (H2O2) under controlled conditions. Application of O-2(-) initially increased maximum Ca2+-activated force but subsequently reduced maximum Ca2+-activated force without altering myofilament Ca2+ sensitivity. Unlike myocardium, caffeine-induced Ca2+ release from the sarcoplasmic reticulum was also inhibited by O-2(-) exposure in diaphragm fibre bundles. Application of H2O2 also increased maximum Ca2+-activated force but had additional effects on resting tension (which increased to 25 % of the control maximum Ca(2+)activated force). H2O2 was without effect on myofilament Ca2+ sensitivitS or caffeine-induced Ca2+ release from the sarcoplasmic reticulum, These data demonstrate that oxidants can potentiate contractile force in the diaphragm through a direct action on the contractile proteins. The potentiation of force is not sustained, however, and under these conditions the detrimental effects of O-2(-) on Ca2+ release from the sarcoplasmic reticulum combined with the effects of oxidants on the contractile proteins mill ultimately compromise excitation-contraction coupling in the diaphragm.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 09:56:43