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Titolo:
The role of mitogen-activated protein kinase activation within focal adhesions in chemotaxis toward FGF-2 by murine brain capillary endothelial cells
Autore:
Shono, T; Kanetake, H; Kanda, S;
Indirizzi:
Nagasaki Univ, Sch Med, Dept Urol, Nagasaki 8528501, Japan Nagasaki Univ Nagasaki Japan 8528501 Dept Urol, Nagasaki 8528501, Japan Nagasaki Univ, Sch Med, Dept Mol Microbiol & Immunol, Nagasaki 8528501, Japan Nagasaki Univ Nagasaki Japan 8528501 & Immunol, Nagasaki 8528501, Japan
Titolo Testata:
EXPERIMENTAL CELL RESEARCH
fascicolo: 2, volume: 264, anno: 2001,
pagine: 275 - 283
SICI:
0014-4827(20010401)264:2<275:TROMPK>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR RECEPTOR-1; SRC FAMILY KINASES; PDGF BETA-RECEPTOR; SIGNAL-TRANSDUCTION; MAP KINASE; TYROSINE KINASES; TRANSGENIC MICE; BINDING-SITE; IN-VIVO; C-FES;
Keywords:
chemotaxis; endothelial cells; FGF-2; focal adhesions; c-Src; MAPK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Kanda, S Nagasaki Univ, Sch Med, Dept Urol, 1-7-1 Sakamoto, Nagasaki 8528501, Japan Nagasaki Univ 1-7-1 Sakamoto Nagasaki Japan 8528501 28501, Japan
Citazione:
T. Shono et al., "The role of mitogen-activated protein kinase activation within focal adhesions in chemotaxis toward FGF-2 by murine brain capillary endothelial cells", EXP CELL RE, 264(2), 2001, pp. 275-283

Abstract

Fibroblast growth factors (FGFs) regulate a number of angiogenic cellular responses such as migration of endothelial cells, To examine the role of mitogen-activated protein kinase (MAPK) in endothelial cell migration, chemotaxis toward FGF-2 was determined in murine brain capillary endothelial cells, denoted IBE cells. PD98059, a specific inhibitor for MAPK/Erk kinase, inhibited FGF-2-induced chemotaxis of IBE cells. It has been reported that c-Src tyrosine kinase phosphorylates focal adhesion kinase at tyrosine 925 within focal adhesions, which in turn creates the binding site for Grb2, leading to MAPK activation. The Src family tyrosine kinase inhibitor, PP1, as well as overexpression of kinase-inactive c-Src, attenuated chemotaxis toward FGF-2. To investigate the signaling events involved in FGF-2-induced chemotaxis, MAPK activation was monitored in IBE cells by indirect immunofluorescence staining. Activated MAPK was initially observed in the cytoplasm andgradually moved into nuclei. A fraction of MAPK was activated by FGF-2 within focal adhesions, where FGF receptor-1 and Src family kinases were also colocalized. MAPK activation within focal adhesions was remarkably decreased in kinase-inactive c-Src-expressing IBE cells. Our data suggest that activation of MAPK by FGF-2 within focal adhesions may depend on c-Src activityand is crucial for FGF-2-induced migration of IBE cells. (C) 2001 AcademicPress.

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Documento generato il 01/12/20 alle ore 08:03:38