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Titolo:
Nitric oxide synthase gene and protein expression are upregulated by Bacille Calmette-Guerin in the rat bladder
Autore:
Oh, BR; Nakajima, K; Ahn, KY; Ryu, SB; Park, YI; Dahiya, R;
Indirizzi:
Vet Adm Med Ctr, Urol Res Ctr 112F, Dept Urol, San Francisco, CA 94121 USAVet Adm Med Ctr San Francisco CA USA 94121 l, San Francisco, CA 94121 USA Univ Calif San Francisco, San Francisco, CA 94121 USA Univ Calif San Francisco San Francisco CA USA 94121 ancisco, CA 94121 USA Chonnam Univ, Sch Med, Dept Urol & Anat, Kwangju 501190, South Korea Chonnam Univ Kwangju South Korea 501190 nat, Kwangju 501190, South Korea Toho Univ, Dept Urol, Tokyo, Japan Toho Univ Tokyo JapanToho Univ, Dept Urol, Tokyo, Japan
Titolo Testata:
EUROPEAN UROLOGY
fascicolo: 3, volume: 39, anno: 2001,
pagine: 349 - 356
SICI:
0302-2838(200103)39:3<349:NOSGAP>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; BCG IMMUNOTHERAPY; CANCER PATIENTS; L-ARGININE; IN-VIVO; INTERLEUKIN-1; NITROTYROSINE; CYTOKINES; THERAPY; DISEASE;
Keywords:
BCG; bladder; iNOS; eNOS; nNOS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Dahiya, R Vet Adm Med Ctr, Urol Res Ctr 112F, Dept Urol, 4150 Clement St, San Francisco, CA 94121 USA Vet Adm Med Ctr 4150 Clement St San Francisco CA USA 94121 1 USA
Citazione:
B.R. Oh et al., "Nitric oxide synthase gene and protein expression are upregulated by Bacille Calmette-Guerin in the rat bladder", EUR UROL, 39(3), 2001, pp. 349-356

Abstract

Objective: We hypothesize that Bacille Calmette-Guerin (BCG) may act through the regulation of various isoforms of nitric oxide synthase (NOS) [inducible (iNOS), endothelial (eNOS), and neuronal (nNOS)] genes and protein expression in rat bladder. Methods: Adult female Sprague-Dawley rats (200-250 g)were injected transurethrally with BCG (22 rats) and phosphate-buffered saline (22 control rats), and after 2, 4, 6, and 12 h, and 1, 2, 3, 5, 7, 10, and 14 days, the bladders were harvested. Normal and BCG-treated rat bladders were analyzed for mRNA expression for iNOS, eNOS, and nNOS by reverse-transcriptase polymerase chain reaction. Protein expression was determined by Western blotting andimmunohistochemistry. Results: mRNA expression for iNOS was induced after 2 h of BCG injection in the rat bladder. Gene expression for iNOS was highest at 6 h to 1 day followed by decreased expression, reaching its lowest level at 5 days. eNOS mRNA expression was detected in control bladders, but its level was higher inthe BCG-treated animals, nNOS mRNA expression was present in all samples, bur did not change after BCG treatment. Western blotting confirmed these findings. Immunohistochemistry analysis demonstrated that eNOS was present mainly in endothelium, while iNOS was detected in stroma and nNOS in epithelium and smooth muscle of the rat bladder. Conclusion: The present study demonstrates, for the first time, that BCG treatment up-regulates gene and protein expression of iNOS and eNOS in normal rat bladders, suggesting that BCG action may be mediated through NOS pathways. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/08/20 alle ore 08:57:22