Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Application of the ethoxyresorufin-O-deethylase (EROD) assay to mixtures of halogenated aromatic compounds
Autore:
Petrulis, JR; Chen, GS; Benn, S; LaMarre, J; Bunce, NJ;
Indirizzi:
Univ Guelph, Dept Chem & Biochem, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 Biochem, Guelph, ON N1G 2W1, Canada Univ Guelph, Dept Biomed Sci, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 omed Sci, Guelph, ON N1G 2W1, Canada
Titolo Testata:
ENVIRONMENTAL TOXICOLOGY
fascicolo: 2, volume: 16, anno: 2001,
pagine: 177 - 184
SICI:
1520-4081(200104)16:2<177:AOTE(A>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIOXIN-LIKE COMPOUNDS; POLYCHLORINATED-BIPHENYLS PCBS; RAT-LIVER MICROSOMES; HEPATIC AH RECEPTOR; DIBENZO-P-DIOXINS; COMPETITIVE-BINDING; CYP1A ACTIVITY; 2,3,7,8-TETRACHLORODIBENZO-PARA-DIOXIN; INHIBITION; METABOLISM;
Keywords:
EROD assay; dioxin; PCBs; aryl hydrocarbon receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Bunce, NJ Univ Guelph, Dept Chem & Biochem, Guelph, ON N1G 2W1, Canada Univ Guelph Guelph ON Canada N1G 2W1 Guelph, ON N1G 2W1, Canada
Citazione:
J.R. Petrulis et al., "Application of the ethoxyresorufin-O-deethylase (EROD) assay to mixtures of halogenated aromatic compounds", ENVIRON TOX, 16(2), 2001, pp. 177-184

Abstract

The ethoxyresorufin-O-deethylase (EROD) assay monitors the induction of the xenobiotic-metabolizing enzyme cytochrome P-450 (CYP) 1A1 and is a widelyused biomarker for exposure of wildlife to substances that bind the aryl hydrocarbon (Ah) receptor. in this work the induction of EROD activity by single compounds and binary mixtures in primary rat hepatocytes was compared with the predictions of a kinetic model involving mixtures of inducers. Theinducing agents were also examined for their ability to activate the Ah receptor to its DNA-binding form and for their ability to act as competitive inhibitors for CYP 1A1. Xenobiotics that failed to activate the Ah receptordid not induce EROD activity. Competitive inhibition for CYP 1A1 between the xenobiotic and 7-ethoxyresorufin caused EROD activity to fall at high xenobiotic concentrations. Competition for a limited number of Ah receptor sites depressed the EROD activity of a strong inducer such as 2,3,7,8-tetrachlorodibenzo-p-dioxin at high concentrations of a weak inducer. Application of the kinetic model to the example of a mixture of low concentrations of dibenzo-p-dioxins and much higher concentrations of polychlorinated biphenyls indicated that EROD assays often seriously underestimate the true potencyof an environmental sample. Hence the EROD assay cannot be used for determining dioxin equivalent concentrations using the toxic equivalence factor approach. (C) 2001 by John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 18:39:45