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Titolo:
The in vitro effect of leptin on growth hormone secretion from primary cultured ovine somatotrophs
Autore:
Chen, C; Roh, SG; Nie, GY; Loneragan, K; Xu, RW; Ruan, M; Clarke, IJ; Goding, JW; Gertler, A;
Indirizzi:
Prince Henrys Inst Med Res, Melbourne, Vic 3168, Australia Prince Henrys Inst Med Res Melbourne Vic Australia 3168 c 3168, Australia Alfred Hosp, Dept Pathol, Prague, Czech Republic Alfred Hosp Prague Czech Republic , Dept Pathol, Prague, Czech Republic Hebrew Univ Jerusalem, Inst Biochem, IL-91905 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91905 -91905 Jerusalem, Israel
Titolo Testata:
ENDOCRINE
fascicolo: 1, volume: 14, anno: 2001,
pagine: 73 - 78
SICI:
1355-008X(200102)14:1<73:TIVEOL>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
GH-RELEASING HORMONE; ANTERIOR-PITUITARY; MESSENGER-RNA; OVARIECTOMIZED EWES; SERUM LEPTIN; NITRIC-OXIDE; RECEPTOR; OBESITY; RAT; EXPRESSION;
Keywords:
leptin; growth hormone; growth hormone-releasing hormone; growth hormone-releasing peptide; receptor; in vitro;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Chen, C Prince Henrys Inst Med Res, POB 5152, Melbourne, Vic 3168, Australia Prince Henrys Inst Med Res POB 5152 Melbourne Vic Australia 3168 a
Citazione:
C. Chen et al., "The in vitro effect of leptin on growth hormone secretion from primary cultured ovine somatotrophs", ENDOCRINE, 14(1), 2001, pp. 73-78

Abstract

Although existing data suggest an influence of leptin on circulating levels of growth hormone (GH), the action site and properties of leptin are still controversial, Using primary cultured ovine pituitary cells, we studied the direct effect of leptin on the secretion of CH, Pituitary cells were dissociated by collagenase and subjected to Percoll gradient centrifugation toenrich the somatotroph population to 60-80% of cells. Treatment of primarycultured ovine somatotrophs with leptin (10(-9)-10(-7) M) for 30 min did not affect basal, GH-releasing hormone (GHRH) (10(-7) M)- or GH-releasing peptide-2 (GHRP-2)(10(-7) M)-stimulated GH secretion. Following treatment of cells for 1-3 d with leptin, GHRH-stimulated GH secretion was reduced and GHRP-2-stimulated GH secretion increased. The combined effect of GHRH and GHRP-2 on GH secretion was not altered by the treatment of cells with leptin for 3 d, GHRH receptor mRNA levels in cultured somatotrophs were decreased but GHRP receptor mRNA levels were increased by 3-d leptin treatment. Theseresults suggest that leptin has a longterm effect on somatotrophs to reduce GHRH receptor synthesis leading to a decrease in GHRH-stimulated GH secretion, Leptin appears, however, to have an opposite effect on GHRP receptor synthesis leading to an increase in GHRP-stimulated GH secretion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:13:58