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Titolo:
Growth hormone secretagogue receptor family members and ligands
Autore:
Smith, RG; Leonard, R; Bailey, ART; Palyha, O; Feighner, S; Tan, C; McKee, KK; Pong, SS; Griffin, P; Howard, A;
Indirizzi:
Baylor Coll Med, Huffington Ctr Aging, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 ton Ctr Aging, Houston, TX 77030 USA Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 Cellular Biol, Houston, TX 77030 USA Merck Res Labs, Rahway, NJ USA Merck Res Labs Rahway NJ USAMerck Res Labs, Rahway, NJ USA
Titolo Testata:
ENDOCRINE
fascicolo: 1, volume: 14, anno: 2001,
pagine: 9 - 14
SICI:
1355-008X(200102)14:1<9:GHSRFM>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PITUITARY CYTOTROPIC FACTOR; MOTILIN BINDING-SITES; FISH FUGU RUBRIPES; GASTROINTESTINAL-TRACT; TYROSINE-HYDROXYLASE; GENOMIC STRUCTURE; ADENOSINE; HEXARELIN; RAT; EXPRESSION;
Keywords:
growth hormone secretagogue receptor; motilin; adenosine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Smith, RG Baylor Coll Med, Huffington Ctr Aging, M320,1 Baylor Plaza, Houston, TX 77030 USA Baylor Coll Med M320,1 Baylor Plaza Houston TX USA 77030 030 USA
Citazione:
R.G. Smith et al., "Growth hormone secretagogue receptor family members and ligands", ENDOCRINE, 14(1), 2001, pp. 9-14

Abstract

We have previously reported the cloning and characterization of a new orphan G-protein-coupled receptor (GPC-R), the growth hormone secretagogue receptor (GHS-R), and shown that this receptor mediates the activity of the growth hormone-releasing peptides (CHRPs) and nonpeptide ligands such as L-692,429 and MK-0677. Because the GHS-R obviously does not belong to any of theknown GPC-R subfamilies, we searched for GHS-R family members by screeninga human genomic library using low-stringency hybridization and screening aPufferfish genomic library. The Pufferfish was selected because of its compact genome. From the human genomic library, a homolog, GPR38, with 52% identity to the GHS-R was isolated. From the Pufferfish library, three family members were isolated. The Pufferfish gene having 58% identity to the GHS-R, on expression in HEK293 cells, was activated with GHRP-6 and MK-0677. These results indicate that the CHS-R has been conserved for at least 400 million years and that the Pufferfish genome is appropriate for isolation of GHS-R family members. In our search for endogenous ligands for the orphan receptors CHS-R and GPR38, we showed that adenosine is a partial agonist of the GHS-R and that motilin is the endogenous ligand for GPR38. We also confirmed that the endogenous ligand ghrelin is a full agonist of the GHS-R.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 10:54:18