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Titolo:
Myf-5 is transiently expressed in nonmuscle mesoderm and exhibits dynamic regional changes within the presegmented mesoderm and somites I-IV
Autore:
Kiefer, JC; Hauschka, SD;
Indirizzi:
Univ Washington, Dept Biochem, Seattle, WA 98195 USA Univ Washington Seattle WA USA 98195 Dept Biochem, Seattle, WA 98195 USA
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 1, volume: 232, anno: 2001,
pagine: 77 - 90
SICI:
0012-1606(20010401)232:1<77:MITEIN>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEURAL CREST CELLS; SKELETAL-MUSCLE; CHICK-EMBRYO; SONIC HEDGEHOG; PRESOMITIC MESODERM; PRECURSOR CELLS; NEURONAL DIFFERENTIATION; PARAXIAL MESODERM; REGULATORY GENE; DELTA-HOMOLOG;
Keywords:
somite; somitogenesis; myogenesis; muscle; Myf-5; MyoD; Delta-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
71
Recensione:
Indirizzi per estratti:
Indirizzo: Hauschka, SD Univ Washington, Dept Biochem, Box 357350, Seattle, WA 98195 USA Univ Washington Box 357350 Seattle WA USA 98195 WA 98195 USA
Citazione:
J.C. Kiefer e S.D. Hauschka, "Myf-5 is transiently expressed in nonmuscle mesoderm and exhibits dynamic regional changes within the presegmented mesoderm and somites I-IV", DEVELOP BIO, 232(1), 2001, pp. 77-90

Abstract

Myf-5 is one of four myogenic regulatory factors that play important rolesin skeletal muscle development. This study provides detailed analysis of Myf-5 expression during early chick development using an in situ hybridization technique that has been optimized to detect low level Myf-5 transcripts. This facilitated detection of heretofore unrecognized dynamic changes in Myf-5 expression patterns. Myf-5 expression is first detected at stage 3 in the primitive streak and exhibits transient low-level expression in nonmyogenic mesoderm. Myf-5 is later expressed in the presegmented mesoderm (psm) in a reiterating pattern that is coordinated with somitogenesis and also colocalizes with the Notch ligand C-Delta-1. In somites (S) I-IV, Myf-5 expression exhibits dynamic regional changes, and in somites rostral to S IV, Myf-5 is expressed at higher levels in muscle precursors in the dorsomedial somite. Semiquantitative comparison of Myf-5 mRNA levels in the psm and in myotome-containing somites indicates about a 10-fold difference. The expression pattern of Myf-5 differs from that of MyoD, which we find is expressed only in the dorsomedial somite. These data reveal that Myf-5 is expressed at low levels several stages before muscle differentiation occurs and suggest that only a subset of cells that initially express Myf-5 will upregulate its expression and differentiate as muscle. (C) 2001 Academic Press.

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Documento generato il 05/07/20 alle ore 06:14:12