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Titolo:
Expression of tissue factor pathway inhibitor 2 inversely correlates during the progression of human gliomas
Autore:
Rao, CN; Lakka, SS; Kin, Y; Konduri, SD; Fuller, GN; Mohanam, S; Rao, JS;
Indirizzi:
Univ Illinois, Coll Med, Div Canc Biol, Dept Biomed & Therapeut Sci, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 d & Therapeut Sci, Peoria, IL 61656 USA Univ Illinois, Coll Med, Div Canc Biol, Dept Neurosurg, Peoria, IL 61656 USA Univ Illinois Peoria IL USA 61656 l, Dept Neurosurg, Peoria, IL 61656 USA Univ Texas, MD Anderson Canc Ctr, Dept Neurosurg, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 tr, Dept Neurosurg, Houston, TX 77030 USA Univ Texas, MD Anderson Canc Ctr, Dept Neuropathol, Houston, TX 77030 USA Univ Texas Houston TX USA 77030 , Dept Neuropathol, Houston, TX 77030 USA
Titolo Testata:
CLINICAL CANCER RESEARCH
fascicolo: 3, volume: 7, anno: 2001,
pagine: 570 - 576
SICI:
1078-0432(200103)7:3<570:EOTFPI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERINE-PROTEASE INHIBITOR; MATRIX-DEGRADING METALLOPROTEINASE; PLASMINOGEN-ACTIVATOR RECEPTOR; ENDOTHELIAL GROWTH-FACTOR; CELL-LINE; PROTEINASE-INHIBITORS; MELANOMA-CELLS; FACTOR VIIA; IN-SITU; UROKINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Rao, JS Univ Illinois, Coll Med, Div Canc Biol, Dept Biomed & Therapeut Sci, 1 Illini Dr, Peoria, IL 61656 USA Univ Illinois 1 Illini Dr Peoria IL USA 61656 Peoria, IL 61656 USA
Citazione:
C.N. Rao et al., "Expression of tissue factor pathway inhibitor 2 inversely correlates during the progression of human gliomas", CLIN CANC R, 7(3), 2001, pp. 570-576

Abstract

Protease inhibitors regulate a variety of physiological and pathological processes including angiogenesis, embryo implantation, intravascular fibrinolysis, mound healing, and tumor invasion. Tissue factor pathway inhibitor (TFPI) 2 is a M-r 32,000 Kunitz-type serine protease inhibitor that inhibitsplasmin, trypsin, chymotrypsin, cathepsin G, and plasma kallikrein but noturokinase-type plasminogen activator, tissue plasminogen activator, or thrombin, In this study, we determined the relative amounts of TFPI-2 in low-,intermediates and high-grade human glioma cell Lines and tumor tissue samples. TFPI-2 protein and mRNA levels (measured by Western and Northern blotting) were highest in low-grade glioma cells (Hs683), lower in anaplastic astrocytoma cells (SW1088 and SW1783), and undetectable In high-grade glioma cells (SNB19). Analysis of TFPI-2 protein in human normal brain and in glioma tumor tissues far TFPI-2 revealed the highest levels in normal brain, lesser amounts in low-grade gliomas and anaplastic astrocytomas, and undetectable amounts in glioblastomas. In situ hybridization of TFPI-2 mRNA with normal brain tissues revealed the greatest positivity in neurons, with moderate positivity in both glial and endothelial cells and moderate, little, or no TFPI-2 mRNA in low-grade glioma, anaplastic astrocytoma, and glioblastoma tumor tissue samples, respectively. We also found that recombinant TFPI-2inhibited the invasive-ness of SNB19 glioblastoma cells in a Matrigel assay in a dose-dependent manner. Collectively, these results suggest that TFPI-2 has a regulatory role in the invasiveness of gliomas irt vitro and in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 07:17:04