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Titolo:
Identification of some rabbit allergens as lipocalins
Autore:
Baker, J; Berry, A; Boscato, LM; Gordon, S; Walsh, BJ; Stuart, MC;
Indirizzi:
Macquarie Univ, Dept Biol Sci, Sydney, NSW 2109, Australia Macquarie UnivSydney NSW Australia 2109 Sci, Sydney, NSW 2109, Australia St Vincents Hosp, Inst Lab Med, Sydney, NSW 2010, Australia St Vincents Hosp Sydney NSW Australia 2010 d, Sydney, NSW 2010, Australia Natl Heart & Lung Inst, Imperial Coll, Sch Med, Dept Occupat & Environm Med, London, England Natl Heart & Lung Inst London England t & Environm Med, London, England Macquarie Univ, Australian Proteome Anal Facil, Sydney, NSW 2109, Australia Macquarie Univ Sydney NSW Australia 2109 cil, Sydney, NSW 2109, Australia
Titolo Testata:
CLINICAL AND EXPERIMENTAL ALLERGY
fascicolo: 2, volume: 31, anno: 2001,
pagine: 303 - 312
SICI:
0954-7894(200102)31:2<303:IOSRAA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
LABORATORY-ANIMAL ALLERGY; ODORANT-BINDING PROTEINS; URINARY PROTEINS; 2-DIMENSIONAL ELECTROPHORESIS; PHEROMONE BINDING; CROSS-REACTIVITY; MAMMALIAN ORIGIN; MUS-MUSCULUS; CDNA CLONING; RAT URINE;
Keywords:
rabbit allergens; RAST; SDS-PAGE; immunoblots; N-terminal sequencing; lipocalins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Stuart, MC Macquarie Univ, Dept Biol Sci, Sydney, NSW 2109, Australia Macquarie Univ Sydney NSW Australia 2109 , NSW 2109, Australia
Citazione:
J. Baker et al., "Identification of some rabbit allergens as lipocalins", CLIN EXP AL, 31(2), 2001, pp. 303-312

Abstract

Background Rabbits are frequently used as laboratory animals or kept as domestic pets. Rabbit serum albumin and a 17-kDa protein referred to as Ory c1 have previously been reported as allergens. Several other allergenic proteins have been recognized by crossed immuno-electrophoresis but have not been characterized. Objective The aim of this study was to characterize the allergenic proteins present in rabbit saliva, urine and fur on the basis of molecular size and, where possible, to determine their amino acid sequences. Methods Extracts from the male New Zealand white rabbit were used for developing specific direct RAST and RAST inhibition assays. Proteins in the extracts were separated by SDS-PAGE and the individual allergens identified byimmunoblotting with serum from rabbit-allergic individuals. The N-termini of four allergens were sequenced. Results Saliva was the most potent extract. In total, 26 protein bands were recognized as allergens in the three extracts: 12 in saliva, seven in urine and seven in fur. Their molecular weights ranged from an 8-kDa species in saliva to an 80-kDa protein in urine. The N terminal sequences of an 18 kDa and a 21-kDa species in saliva, were identified as lipocalins with sequence similarity to a recently described odourant binding protein. Conclusions This is the first evidence that allergens from the rabbit are members of the lipocalin superfamily of proteins, suggesting that similar mechanisms may be involved in eliciting the allergic response to rabbits. The 18 kDa allergen from saliva may be the previously named rabbit allergen, Ory c 1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 22:57:06