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Titolo:
Simultaneous analysis of sarin, pyridostigmine bromide and their metabolites in rat plasma and urine using HPLC
Autore:
Abu-Qare, W; Abou-Donia, B;
Indirizzi:
Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Pharmacol & Canc Biol, Durham, NC 27710 USA
Titolo Testata:
CHROMATOGRAPHIA
fascicolo: 5-6, volume: 53, anno: 2001,
pagine: 251 - 255
SICI:
0009-5893(200103)53:5-6<251:SAOSPB>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
AGENT DEGRADATION PRODUCTS; CHEMICAL WARFARE AGENTS; NERVE AGENTS; METHYLPHOSPHONIC ACIDS; CHROMATOGRAPHIC METHOD; QUANTITATIVE-ANALYSIS; HYDROLYSIS PRODUCTS; MASS-SPECTROMETRY; PHARMACOKINETICS; ELECTROPHORESIS;
Keywords:
column liquid chromatography; sarin; pyridostigmine bromide; rat plasma and urine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Abou-Donia, B Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 nc Biol, Durham, NC 27710 USA
Citazione:
W. Abu-Qare e B. Abou-Donia, "Simultaneous analysis of sarin, pyridostigmine bromide and their metabolites in rat plasma and urine using HPLC", CHROMATOGR, 53(5-6), 2001, pp. 251-255

Abstract

A method was developed for the separation and quantification of the warfare nerve agent sarin (O-isopropylmethylphosphonoflouridate), its metabolite methylphosphonic acid, the anti nerve agent drug pyridostigmine bromide (PB;3-dimethylaminocarbonyloxy-N-methyl pyridinium bromide) and its metaboliteN-methyl-3-hydroxypyridinium bromide in rat plasma and urine. The method involved using solid phase extraction and high performance liquid chromatography (HPLC) with reversed phase C-18 column, and UV detection at 280 nm. the compounds were separated using gradient of 1% to 55% acetonitrile in 0.1%triflouroacetic acid water solution (pH 3.20) at flow rate of 0.9 ml/min in a period of 15 min. The retention times ranged from 4.4 - 12.1 min. The limits of detection were 50 ng mL(-1) for PB and N-methyl-3-hydroxypyridinivm bromide, and 10 mug ml- I for sarin and methylphosphonic acid, while limits of quantitation were between 100 ng mL(-1) - 12 mug mL(-1). Average percentage recovery of five spiked samples from plasma were 84.6 +/- 8.4, 86.5 /- 9.0, 76.4 +/- 8.5, 81.3 +/- 8.2, and from urine 78.5 +/- 7.9, 76.4 +/- 78, 74.4 +/- 8.4, 80.6 +/- 6.8 for sarin, methylphosphonic acid, pyridostigmine bromide and N-methyl-3-hydroxypyridinium bromide, respectively. This method was applied to analyze the above chemicals and metabolites following combined administration in rats.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 22:55:00