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Titolo:
HCO3- ions modify the role of PKC isoforms in the modulation of rat mast cell functions
Autore:
Vilarino, N; de la Rosa, LA; Vieytes, MR; Botana, LM;
Indirizzi:
Univ Santiago de Compostela, Fac Vet, Dept Farmacol, Lugo 27002, Spain Univ Santiago de Compostela Lugo Spain 27002 Farmacol, Lugo 27002, Spain Univ Santiago de Compostela, Fac Vet, Dept Fisiol, Lugo 27002, Spain Univ Santiago de Compostela Lugo Spain 27002 t Fisiol, Lugo 27002, Spain
Titolo Testata:
CELLULAR SIGNALLING
fascicolo: 3, volume: 13, anno: 2001,
pagine: 177 - 190
SICI:
0898-6568(200103)13:3<177:HIMTRO>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; CAPACITATIVE CALCIUM-ENTRY; EXERCISE-INDUCED ANAPHYLAXIS; BASOPHILIC RBL-2H3 CELLS; JURKAT T-CELLS; CA2+ ENTRY; FEEDBACK-REGULATION; HISTAMINE-RELEASE; XENOPUS OOCYTES; PHORBOL ESTER;
Keywords:
mast cell; thapsigargin; protein kinase C; calcium entry; PKC isoforms; ionophore A23187; PMA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Botana, LM Univ Santiago de Compostela, Fac Vet, Dept Farmacol, Lugo 27002, Spain Univ Santiago de Compostela Lugo Spain 27002 ugo 27002, Spain
Citazione:
N. Vilarino et al., "HCO3- ions modify the role of PKC isoforms in the modulation of rat mast cell functions", CELL SIGNAL, 13(3), 2001, pp. 177-190

Abstract

PKC and the intracellular calcium signal are two well-known intracellular signaling pathways implicated in the induction of mast cell exocytosis. Both signals are modified by the presence or absence of HCO3- ions in the external medium. In this work, we studied the regulation of the exocytotic process by PKC isozymes and its relationship with HCO3- ions and PKC modulationof the calcium entry. The calcium entry, induced by thapsigargin and further addition of calcium, was inhibited by PMA, a PKC activator, and enhancedby 500 nM GF109203X, which inhibits Ca2+-independent PKC isoforms. PMA inhibition of the Ca2+ entry was reverted by 500 and 50 nM GF109203X, which inhibit Ca2+-independent and Ca2+-dependent isoforms, respectively, and Go6976, a specific inhibitor of Ca2+-dependent PKCs. Thus. activation of Ca2+-dependent and Ca2+-independent PKC isoforms inhibit Ca2+ entry in rat mast cells, either in a HCO3--buffered or a HCO3--free medium. PMA, GF109203X, Go6976 and rottlerin, a specific inhibitor of PKC delta, were also used to study the role of PKC isoforms in the regulation of exocytosis induced by thapsigargin. ionophore A23187 and PMA. The results demonstrate that Ca2+-dependent PKC isoforms inhibit exocytosis in a HCO3--dependent way. Moreover, Ca2+-independent PKC delta was the main isoform implicated in promotion of Ca2+-dependent roast cell exocytosis in the presence or absence of HCO3. The role of PKC isoforms in the regulation of mast cell exocytosis depends on the stimulus and on the presence or absence of HCO3- ions in the medium, butit is independent of PKC modulation of the Ca2+ entry. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 09:46:37