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Titolo:
Essential role of the VirB machinery in the maturation of the Brucella abortus-containing vacuole
Autore:
Comerci, DJ; Martinez-Lorenzo, MJ; Sieira, R; Gorvel, JP; Ugalde, RA;
Indirizzi:
Univ Nacl Gen San Martin, CONICET, Inst Invest Biotecnol, RA-1650 Buenos Aires, DF, Argentina Univ Nacl Gen San Martin Buenos Aires DF Argentina RA-1650 DF, Argentina Comis Nacl Energia Atom, Div Agropecuaria, Ctr Atom Ezeiza, RA-1804 BuenosAires, DF, Argentina Comis Nacl Energia Atom Buenos Aires DF Argentina RA-1804 , DF, Argentina CNRS Marseille Luminy, INSERM, Ctr Immunol, F-13288 Marseille, France CNRSMarseille Luminy Marseille France F-13288 F-13288 Marseille, France
Titolo Testata:
CELLULAR MICROBIOLOGY
fascicolo: 3, volume: 3, anno: 2001,
pagine: 159 - 168
SICI:
1462-5814(200103)3:3<159:EROTVM>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTERIUM LEGIONELLA-PNEUMOPHILA; TYPHIMURIUM-CONTAINING VACUOLES; AGROBACTERIUM-TUMEFACIENS VIRB; PHAGOSOME-LYSOSOME FUSION; NUCLEOTIDE-BINDING SITE; INTRACELLULAR-LOCALIZATION; NONPROFESSIONAL PHAGOCYTES; ENDOPLASMIC-RETICULUM; BORDETELLA-PERTUSSIS; VIRULENCE SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Ugalde, RA Univ Nacl Gen San Martin, CONICET, Inst Invest Biotecnol, Ave Gen Paz Entre Constituyentes & Albarellos, RA-1650 Buenos Aires, DF, Argentina Univ Nacl Gen San Martin Ave Gen Paz Entre Constituyentes & Albarellos Buenos Aires DF Argentina RA-1650
Citazione:
D.J. Comerci et al., "Essential role of the VirB machinery in the maturation of the Brucella abortus-containing vacuole", CELL MICROB, 3(3), 2001, pp. 159-168

Abstract

In epithelial cells, the intracellular pathogen Brucella abortus escapes from the endocytic pathway, exploits the autophagic machinery of the host cell and establishes a unique replication niche in the endoplasmic reticulum. The molecular mechanisms underlying these processes are still poorly understood. Recently, a B. abortus type IV-related secretion system encoded by the virB operon has been described as being involved in the intracellular trafficking of the bacteria. In this study, we have analysed the intracellular pathway of B, abortus virB10 mutant strains by confocal microscopy, We demonstrate that a functional virB operon is essential for the biogenesis of the Brucella-containing vacuole, Polar mutation preventing the transcription of virB10 and downstream sequences did not allow Brucella to bypass the endocytic pathway. Consequently, polar mutant-containing vacuoles fused withlysosomes in which bacteria underwent a degradation process. In contrast, virB10 non-polar mutants were capable of avoiding interactions with the endocytic pathway but, diverging to wild-type Brucella, were unable to reach the endoplasmic reticulum to establish their intracellular replication nicheand seemed to be recycled to the cell surface. Based on the two particularphenotypes described in this work, a model of maturation of the Brucella-containing vacuole is proposed.

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Documento generato il 20/10/20 alle ore 05:38:36