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Titolo:
SERCA activity is required for timely progression through G1/S
Autore:
Simon, VR; Moran, MF;
Indirizzi:
Univ Toronto, Charles H Best Inst, Banting & Best Dept Med Res, Toronto, ON M5G 1L6, Canada Univ Toronto Toronto ON Canada M5G 1L6 d Res, Toronto, ON M5G 1L6, Canada
Titolo Testata:
CELL PROLIFERATION
fascicolo: 1, volume: 34, anno: 2001,
pagine: 15 - 30
SICI:
0960-7722(200102)34:1<15:SAIRFT>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-PROLIFERATION; HUMAN FIBROBLASTS; GROWTH-FACTOR; CYCLIN D1; RETICULUM CA2+-ATPASES; EXTRACELLULAR-MATRIX; INTRACELLULAR CA-2+; PHASE-TRANSITION; CALCIUM INFLUX; THAPSIGARGIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Simon, VR Univ Toronto, Charles H Best Inst, Banting & Best Dept Med Res, 112 Coll St,Room 117, Toronto, ON M5G 1L6, Canada Univ Toronto 112 Coll St,Room 117 Toronto ON Canada M5G 1L6 nada
Citazione:
V.R. Simon e M.F. Moran, "SERCA activity is required for timely progression through G1/S", CELL PROLIF, 34(1), 2001, pp. 15-30

Abstract

Changes in intracellular Ca2+ correlate with specific events in the cell cycle. Here we investigated the role of Ca2+ in the G1 phase. HEK 293 cells were arrested in mitosis and subjected to short-term treatments that alter Ca2+ homeostasis prior to their release into G1. Treatment with thapsigargin (TG), an irreversible inhibitor of the sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) lengthened the G1 phase. Moreover, TG treatment also resultedin a dramatic alteration in cellular morphology and attachment and in the reduction of MAPK activity and lower levels of cyclin D1 and cyclin E proteins. Treatments with reagents that transiently increase or decrease cytosolic Ca2+ or that temporarily inactivate SERCA did not alter any of the aboveparameters. Cells expressing a TG-resistant form of SERCA progressed normally through the G1/S transition after TG treatment. These results suggest that long-term SERCA inactivation affects cell cycle-dependent events and compromises progression through G1/S.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 18:07:12