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Titolo:
Lysosomal enzymes in the macronucleus of Tetrahymena during its apoptosis-like degradation
Autore:
Lu, E; Wolfe, J;
Indirizzi:
Wesleyan Univ, Dept Biol, Middletown, CT 06459 USA Wesleyan Univ Middletown CT USA 06459 Dept Biol, Middletown, CT 06459 USA
Titolo Testata:
CELL DEATH AND DIFFERENTIATION
fascicolo: 3, volume: 8, anno: 2001,
pagine: 289 - 297
SICI:
1350-9047(200103)8:3<289:LEITMO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; NUCLEAR DEATH; DNA FRAGMENTATION; DIFFERENTIATION; AUTOPHAGOSOMES; DEGENERATION; THERMOPHILA; MICRONUCLEI; MECHANISMS; ACTIVATION;
Keywords:
acidification; apoptosis; autophagosome; ciliate; lysosome; nucleus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Wolfe, J Wesleyan Univ, Dept Biol, Middletown, CT 06459 USA Wesleyan UnivMiddletown CT USA 06459 , Middletown, CT 06459 USA
Citazione:
E. Lu e J. Wolfe, "Lysosomal enzymes in the macronucleus of Tetrahymena during its apoptosis-like degradation", CELL DEAT D, 8(3), 2001, pp. 289-297

Abstract

A key characteristic of apoptosis is its regulated nuclear degradation. Apoptosis-like nuclear degradation also occurs in the ciliated unicellular organism, Tetrahymena thermophila. Chromatin of the macronucleus undergoes massive condensation, a process that can be blocked by caspase inhibitors. The nucleus becomes TUNEL-positive, and its DNA is cleaved into nucleosome-sized fragments, in a matter of hours the macronucleus is completely degraded, and disappears. The condensed nucleus sequesters acridine orange, which means that it might become an acidic compartment. We therefore asked whetherlysosomal bodies fuse with the condensed macronucleus to form an autophagosome. We monitored acid phosphatase (AP) activity, which is associated withlysosomal bodies but is not found in normal nuclei. We find that after themacronucleus condenses AP activity is localized in cap-like structures at its cortex. Later, after the degrading macronucleus loses much of its DNA, acid phosphatase deposits appear deeper within the nucleus. We conclude that although macronuclear elimination is initiated by an apoptosis-like mechanism, its final degradation may be achieved through autophagosomy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 09:59:53