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Titolo:
p53-dependent apoptosis induced by proteasome inhibition in mammary epithelial cells
Autore:
MacLaren, AP; Chapman, RS; Wyllie, AH; Watson, CJ;
Indirizzi:
Western Gen Hosp, Mol Med Ctr, Sir Alastair Currie CRC Labs, Edinburgh EH24XU, Midlothian, Scotland Western Gen Hosp Edinburgh Midlothian Scotland EH2 4XU dlothian, Scotland Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England Univ Cambridge Cambridge England CB2 1QP hol, Cambridge CB2 1QP, England
Titolo Testata:
CELL DEATH AND DIFFERENTIATION
fascicolo: 3, volume: 8, anno: 2001,
pagine: 210 - 218
SICI:
1350-9047(200103)8:3<210:PAIBPI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-DAMAGE; DEATH; CYCLE; PATHWAY; P53; INVOLVEMENT; DEGRADATION; ACTIVATION; PROGRAM;
Keywords:
KIM-2 mammary epithelial cells; p53; apoptosis; 26S proteasome;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: MacLaren, AP Beatson Inst Canc Res, CRC, Garscube Estate,Switchback Rd, Glasgow G61 1BD, Lanark, Scotland Beatson Inst Canc Res Garscube Estate,Switchback Rd Glasgow Lanark Scotland G61 1BD
Citazione:
A.P. MacLaren et al., "p53-dependent apoptosis induced by proteasome inhibition in mammary epithelial cells", CELL DEAT D, 8(3), 2001, pp. 210-218

Abstract

We have examined the effects of inhibition of the 26S proteasome in a murine mammary cell line, KIM-2 cells using the peptide aldehyde inhibitor MG132, These studies have demonstrated a clear requirement for proteasome function in cell viability. Induction of apoptosis was observed following MG132 treatment in KIM-2 cells and this death was shown to be dependent on the cell actively traversing the cell cycle. KIM-2 cells were generated using a temperature sensitive T-antigen (Tag) and studies at the permissive temperature (33 C) have shown that a Tag binding protein was essential for this apoptotic response, Studies in two additional cell lines, HC11, which is a mammary epithelial cell line carrying mutant p53 alleles and p53 null ES cellssuggest that p53 is actively required for the apoptosis induced as a consequence of proteasome inhibition, These results suggest a pivotal role for the 26S proteasome degradation pathway in progression through the cell cyclein proliferating cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 07:32:46