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Titolo:
Trisomy 8 and monosomy 7 detected in bone marrow using primed in situ labeling, fluorescence in situ hybridization, and conventional cytogenetic analyses. A study of 54 cases with hematological disorders
Autore:
Yan, J; Zhang, XX; Fetni, R; Drouin, R;
Indirizzi:
Univ Laval, Fac Med, Dept Med Biol, Div Pathol, Quebec City, PQ G1L 3L5, Canada Univ Laval Quebec City PQ Canada G1L 3L5 Quebec City, PQ G1L 3L5, Canada CHUQ, Hop St Francois Assie, Unite Rech & Genet Humaine & Mol, Quebec City, PQ G1L 3L5, Canada CHUQ Quebec City PQ Canada G1L 3L5 & Mol, Quebec City, PQ G1L 3L5, Canada Duke Univ, Ctr Med, Dept Pathol, Durham, NC USA Duke Univ Durham NC USADuke Univ, Ctr Med, Dept Pathol, Durham, NC USA Duke Univ, Ctr Med, Dept Pathol, Van Nuys, CA 91405 USA Duke Univ Van Nuys CA USA 91405 Med, Dept Pathol, Van Nuys, CA 91405 USA Smithkline Beecham Clin Labs, Van Nuys, CA 91405 USA Smithkline Beecham Clin Labs Van Nuys CA USA 91405 Van Nuys, CA 91405 USA Hop Maisonneuve Rosemont, Dept Hematol, Lab Cytogenet, Montreal, PQ H1T 2M4, Canada Hop Maisonneuve Rosemont Montreal PQ Canada H1T 2M4 l, PQ H1T 2M4, Canada
Titolo Testata:
CANCER GENETICS AND CYTOGENETICS
fascicolo: 1, volume: 125, anno: 2001,
pagine: 30 - 40
SICI:
0165-4608(200102)125:1<30:T8AM7D>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC LYMPHOCYTIC-LEUKEMIA; ACUTE MYELOID-LEUKEMIA; IN-SITU; INTERPHASE CYTOGENETICS; HUMAN-CHROMOSOMES; HUMAN SPERM; MYELODYSPLASTIC SYNDROMES; PROLIFERATIVE ADVANTAGE; INSITU HYBRIDIZATION; PRINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Drouin, R Univ Laval, Fac Med, Dept Med Biol, Div Pathol, 10 Espinay, Quebec City, PQ G1L 3L5, Canada Univ Laval 10 Espinay Quebec City PQ Canada G1L3L5 3L5, Canada
Citazione:
J. Yan et al., "Trisomy 8 and monosomy 7 detected in bone marrow using primed in situ labeling, fluorescence in situ hybridization, and conventional cytogenetic analyses. A study of 54 cases with hematological disorders", CANC GENET, 125(1), 2001, pp. 30-40

Abstract

Trisomy 8 and monosomy 7 are the two most frequent aneuploidies found in hematological disorders such as myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). In this study, primed in situ labeling (PRINS), fluorescence in situ hybridization (FISH) and conventional cytogenetic approacheswere used to investigate 54 cases of hematopoietic disorders. Of these cases, there were 22 cases of trisomy 8, 2 cases of tetrasomy 8, 14 cases of monosomy 7, and 16 cases with two copies of both chromosomes 7 and 8. PRINS was carried out in interphase nuclei of bone marrow samples using primers that can specifically detect alpha -satellite DNA sequences of chromosomes 7and S. In parallel, using the alpha -satellite probes for chromosomes 7 and 8, FISH was performed for all the cases. PRINS and FISH techniques showedsimilar specificity and sensitivity. In comparison to FISH, PRINS is more advantageous since it is a faster, easier, and more cost-effective technique. Additionally, for most of the cases, a higher proportion of aneuploidy was detected in metaphases using conventional cytogenetics, as compared to the one found in interphase nuclei identified with PRINS and FISH techniques. In other words, for these cases, the cells with trisomy 8 or monosomy 7, had a distinct proliferative advantage compared to the disomic cell population. Therefore, to better quantify the proportion of aneuploid cells in bane marrow, we recommend to investigate the frequency of aneuploidy in nucleiusing PRINS, rather than studying only the dividing cells as detected by conventional cytogenetic techniques. (C) 2001 Elsevier Science me. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 22:40:49