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Titolo:
Tumour-amplified kinase BTAK is amplified and overexpressed in gastric cancers with possible involvement in aneuploid formation
Autore:
Sakakura, C; Hagiwara, A; Yasuoka, R; Fujita, Y; Nakanishi, M; Masuda, K; Shimomura, K; Nakamura, Y; Inazawa, J; Abe, T; Yamagishi, H;
Indirizzi:
Kyoto Prefectural Univ Med, Dept Digest Surg, Kamigyo Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med, Dept Hyg, Kamigyo Ku, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kyoto Japan 6028566 Ku, Kyoto 6028566, Japan Univ Tokyo, Inst Med Sci, Ctr Human Genome, Minato Ku, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 n Genome, Minato Ku, Tokyo 1088639, Japan
Titolo Testata:
BRITISH JOURNAL OF CANCER
fascicolo: 6, volume: 84, anno: 2001,
pagine: 824 - 831
SICI:
0007-0920(20010323)84:6<824:TKBIAA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPARATIVE GENOMIC HYBRIDIZATION; LEUKEMIC HL-60 CELLS; BREAST-CANCER; DNA; AMPLIFICATION; EXPRESSION; GENE; DIFFERENTIATION; APOPTOSIS; RECEPTOR;
Keywords:
ploidy pattern; amplification; overexpression; BTAK; gastric cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Sakakura, C Kyoto Prefectural Univ Med, Dept Digest Surg, Kamigyo Ku, Kawaramachi Dori, Kyoto 6028566, Japan Kyoto Prefectural Univ Med Kawaramachi Dori Kyoto Japan 6028566
Citazione:
C. Sakakura et al., "Tumour-amplified kinase BTAK is amplified and overexpressed in gastric cancers with possible involvement in aneuploid formation", BR J CANC, 84(6), 2001, pp. 824-831

Abstract

Our recent analysis of gastric cancers using comparative genomic hybridization (CGH) revealed a novel high frequent copy number increase in the long arm of chromosome 20. Tumour-amplified kinase BTAK was recently cloned frombreast cancers and mapped on 20q13 as a target gene for this amplificationin human breast cancers. In the study presented here, we analysed BTAK copy-number and expression, and their relation to the ploidy pattern in 72 primary gastric cancers. Furthermore, wild-type BTAK and its deletion mutants were transfected to gastric cancers to examine changes in cell proliferation and DNA ploidy pattern. Evaluation of 72 unselected primary gastric cancers found BTAK amplification in 5% and overexpression in more than 50%. All four clinical samples with BTAK amplification showed aneuploidy and poor prognosis. Transfection of BTAK in near-diploid gastric cancers induced another aneuploid cell population. In contrast, the c-terminal-deleted mutant ofBTAK induced no effect in DNA ploidy pattern and inhibited gastric cancer cell proliferation. These results suggest that BTAK may be involved in gastric cancer cell aneuploid formation, and is a candidate gene for the increase in the number of copies of the 20q, and thus may contribute to an increase in the malignant phenotype of gastric cancer. (C) 2001 Cancer Research Campaign http://www, bjcancer.com.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 18:25:36