Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Agonists determine the pattern of G-protein activation in mu-opioid receptor-mediated supraspinal analgesia
Autore:
Sanchez-Blazquez, P; Gomez-Serranillos, P; Garzon, J;
Indirizzi:
CSIC, Inst Cajal, E-28002 Madrid, Spain CSIC Madrid Spain E-28002CSIC, Inst Cajal, E-28002 Madrid, Spain Univ Complutense Madrid, Fac Farm, Madrid, Spain Univ Complutense Madrid Madrid Spain se Madrid, Fac Farm, Madrid, Spain
Titolo Testata:
BRAIN RESEARCH BULLETIN
fascicolo: 2, volume: 54, anno: 2001,
pagine: 229 - 235
SICI:
0361-9230(20010115)54:2<229:ADTPOG>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTISENSE OLIGODEOXYNUCLEOTIDES; TRANSDUCER PROTEINS; MORPHINE-6-BETA-GLUCURONIDE ANALGESIA; ALPHA-SUBUNITS; BINDING; HEROIN; MICE; SELECTIVITY; EFFICACY; ANTINOCICEPTION;
Keywords:
mu-opioid agonists; GTP-binding regulatory proteins; opioid receptor-G-protein complexes; antisenseoligodeoxynucleotides;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Sanchez-Blazquez, P CSIC, Inst Cajal, Ave Doctor Arce 37, E-28002 Madrid, Spain CSIC Ave Doctor Arce 37 Madrid Spain E-28002 d, Spain
Citazione:
P. Sanchez-Blazquez et al., "Agonists determine the pattern of G-protein activation in mu-opioid receptor-mediated supraspinal analgesia", BRAIN RES B, 54(2), 2001, pp. 229-235

Abstract

The opioids heroin, methadone, buprenorphine, and morphine produce supraspinal antinociception in CD-1 mice that is antagonized by Cys(2), Tyr(3), Om(5), Pen(7)-amide but not by naltrindole or nor-binaltorphimine. The patterns of GTP-binding regulatory proteins (G-proteins) activation exhibited by these agonists at mu -opioid receptors were characterized. The expression of alpha -subunits of Gi-protein classes, Gi1, Gi2, Gi3, Go1, Go2 and Gz, and those of the Gq-protein family, Gq and G11, was reduced by administrationof antisense oligodeoxynucleotides (ODNs) complementary to sequences in their respective mRNAs. The ODN treatments demonstrated differences in the analgesic profiles of these opioids. Though the knock-down of G(i2)alpha or G(z)alpha subunits diminished the analgesic effets of the four opioids, impairment of G(i3)alpha did not modify the potency of morphine. In mice with reduced G(i1)alpha, G(o1)alpha or G(11)alpha levels, antinociception inducedby heroin and methadone was diminished, but buprenorphine and morphine showed no change in their effects. Also, antinociception induced by heroin andbuprenorphine, but neither morphine nor methadone, required intact G(o2)alpha or G(q)alpha levels. Thus, morphine, heroin, methadone, and buprenorphine showed different patterns of G-protein activation in evoking p-opioid receptor-mediated supraspinal antinociception. Therefore, after binding identical receptors, each agonist determines the classes of GTP-binding regulatory transducer proteins to be activated. (C) 2001 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 21:46:48