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Titolo:
Possible role of enhanced microtubule phosphorylation in dichlorvos induced delayed neurotoxicity in rat
Autore:
Choudhary, S; Joshi, K; Gill, KD;
Indirizzi:
Postgrad Inst Med Educ & Res, Dept Biochem, Chandigarh 160012, India Postgrad Inst Med Educ & Res Chandigarh India 160012 igarh 160012, India Postgrad Inst Med Educ & Res, Dept Histopathol, Chandigarh 160012, India Postgrad Inst Med Educ & Res Chandigarh India 160012 igarh 160012, India
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 897, anno: 2001,
pagine: 60 - 70
SICI:
0006-8993(20010406)897:1-2<60:PROEMP>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHOROFLUORIDATE (DFP)-TREATED HEN; ORTHO-CRESYL PHOSPHATE; NEUROPATHY TARGET ESTERASE; PROTEIN-KINASE-II; BRAIN SUPERNATANT; NERVOUS-SYSTEM; SPINAL-CORD; TUBULIN; CALCIUM; OPIDN;
Keywords:
dichlorvos; delayed neurotoxicity; microtubule; phosphorylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Gill, KD Postgrad Inst Med Educ & Res, Dept Biochem, Chandigarh 160012, India Postgrad Inst Med Educ & Res Chandigarh India 160012 012, India
Citazione:
S. Choudhary et al., "Possible role of enhanced microtubule phosphorylation in dichlorvos induced delayed neurotoxicity in rat", BRAIN RES, 897(1-2), 2001, pp. 60-70

Abstract

The effect of a single subcutaneous dose of 200 mg/kg body weight dichlorvos on neuronal microtubule phosphorylation has been studied in rat following the development of organophosphate induced delayed neurotoxicity (OPIDN). Microtubule associated Ca2+/ calmodulin dependent as well as cAMP dependent protein kinases were assayed. Dichlorvos administration led to a consistent increase in the activity of both the kinases at an post exposure intervals (7th, 15th and 21st day) as compared to that of controls. After in vitrophosphorylation using [gamma-P-32]ATP, various proteins were resolved on one-dimensional 8% SDS-PAGE, stained with Coomassie Blue and autoradiographed. The amount of P-32 incorporated was quantified by microdensitometry. Dichlorvos enhanced the phosphorylation of 55- and 280-kDa proteins. These twoproteins were identified as tubulin and microtubule associated protein-2 (MAP-2) by immunoblotting. This study showed that dichlorvos induced hyperphosphorylation of tubulin and MAP-2 which in turn destabilizes microtubule assembly, and may ultimately result in axonal degeneration leading to dichlorvos induced delayed neurotoxicity. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 29/09/20 alle ore 12:52:52