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Titolo:
The effect of macrophage-colony stimulating factor and other humoral factors (interleukin-1,-3,-6, and-11, tumor necrosis factor-alpha, and granulocyte macrophage-colony stimulating factor) on human osteoclast formation fromcirculating cells
Autore:
Fujikawa, Y; Sabokbar, A; Neale, SD; Itonaga, I; Torisu, T; Athanasou, NA;
Indirizzi:
Univ Oxford, Nuffield Dept Orthopaed Surg, Oxford, England Univ Oxford Oxford England uffield Dept Orthopaed Surg, Oxford, England Oita Med Univ, Dept Orthoped Surg, Oita, Japan Oita Med Univ Oita JapanOita Med Univ, Dept Orthoped Surg, Oita, Japan
Titolo Testata:
BONE
fascicolo: 3, volume: 28, anno: 2001,
pagine: 261 - 267
SICI:
8756-3282(200103)28:3<261:TEOMSF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
BONE-MARROW CULTURES; DIFFERENTIATION FACTOR; STROMAL CELLS; MOUSE; RECEPTOR; OSTEOPETROSIS; OSTEOBLASTS; SURVIVAL; MUTATION; GROWTH;
Keywords:
human osteoclasts; monocytes; M-CSF; IL-3; GM-CSF; bone resorption;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Athanasou, NA Nuffield Orthopaed Ctr, Dept Pathol, Windmill Rd, Oxford OX37LD, England Nuffield Orthopaed Ctr Windmill Rd Oxford England OX3 7LD d
Citazione:
Y. Fujikawa et al., "The effect of macrophage-colony stimulating factor and other humoral factors (interleukin-1,-3,-6, and-11, tumor necrosis factor-alpha, and granulocyte macrophage-colony stimulating factor) on human osteoclast formation fromcirculating cells", BONE, 28(3), 2001, pp. 261-267

Abstract

Macrophage-colony stimulating factor (M-CSF) is an essential requirement for human osteoclast formation, but its effect on the proliferation and differentiation of circulating osteoclast precursor cells is unknown. Other growth factors and cytokines are also known to support/stimulate osteoclast formation fi om mouse marrow precursors, but it is not certain whether those factors similarly influence human osteoclast formation. In this study, human monocytes were cocultured with osteoblast-like UMR-106 cells on coverslips and dentine slices for up to 21 days in the presence of 1,25 dihydroxyvitamin D-3 (10(-7) mol/L), dexamethasone (10(-8) mol/L), and various concentrations of either M-CSF or other humoral factors (interleukin [IL]-1 beta, IL-3, IL-6, and IL-11; tumor necrosis fartor-alpha [TNF-alpha]; and granulocyte macrophage [GM]-CSF). The effect on osteoclast formation,vas assessed by tartrate-resistant acid phosphatase (TRAP) and vitronectin receptor staining and lacunar bone resorption. The results of time-course and proliferation studies showed that M-CSF stimulated both the proliferative and differentiation stages of human osteoclast formation from circulating osteoclast precursors in a dose-dependent manner. A high concentration of M-CSF (100 ng/mL) did not inhibit osteoclast formation. IL-3 and GM-CSF were also capableof stimulating human osteoclast formation, although these growth factors were much less potent than M-CSF. IL-3- and GM-CSF-stimulated osteoclast formation was inhibited by an antibody specific for human M-CSF. Osteoclast formation and lacunar resorption was not seen when either TNF-alpha, IL-1 beta, IL-6 (+ soluble IL-6 receptor), or IL-11 was substituted for M-CSF during coculture, Those results confirm that CII-CSF is essential for human osteoclast formation from circulating mononuclear precursors, and also show's that IL-3 and GM-CSF may support osteoclast differentiation via the stimulation of M-CSF production by human monocytes. (Bone 28:261-267; 2001) (C) 2001 by Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/02/20 alle ore 08:28:05