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Titolo:
Proliferation and differentiation of prostatic stromal cells
Autore:
Niu, Y; Xu, Y; Zhang, J; Bai, J; Yang, H; Ma, T;
Indirizzi:
Tianjin Inst Urol Surg, Tianjin 300211, Peoples R China Tianjin Inst Urol Surg Tianjin Peoples R China 300211 1, Peoples R China Nankai Univ, Tianjin 300071, Peoples R China Nankai Univ Tianjin PeoplesR China 300071 anjin 300071, Peoples R China Tianjin Electron Microscopy Ctr, Tianjin, Peoples R China Tianjin ElectronMicroscopy Ctr Tianjin Peoples R China Peoples R China
Titolo Testata:
BJU INTERNATIONAL
fascicolo: 4, volume: 87, anno: 2001,
pagine: 386 - 393
SICI:
1464-4096(200103)87:4<386:PADOPS>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELL; FIBROBLAST GROWTH-FACTOR; ESTROGEN; EXPRESSION; ANDROGEN; HYPERPLASIA; INDUCTION; PHENOTYPE; LOBES;
Keywords:
prostate; castration; stromal cells; differentiation; growth factors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Niu, Y Tianjin Inst Urol Surg, Pingjiang Rd 23, Tianjin 300211, Peoples R China Tianjin Inst Urol Surg Pingjiang Rd 23 Tianjin Peoples R China 300211
Citazione:
Y. Niu et al., "Proliferation and differentiation of prostatic stromal cells", BJU INT, 87(4), 2001, pp. 386-393

Abstract

Objectives To investigate the effects of androgen, transforming growth factor beta1 (TGF-beta) and basic fibroblast growth factor (bFGF) on the proliferation and differentiation of prostatic stromal cells of the dog in vivo and human stromal cells in vitro. Materials and methods Twenty-two dogs had their serum concentration of testosterone and oestradiol determined by radioimmunoassay before and after castration. Light microscopy, transmission electron microscopy and an in situcell-death assay were carried out successively before and after castrationto evaluate prostatic histomorphology. A semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was used to evaluate the expression of TGF-beta, bFGF and myosin in the canine prostate tissue after castration. In vitro serum-free cell cultures from human prostatic stroma were established and exposed to dihydrotestosterone (DHT), TGF-beta and bFGF in various concentrations. The proliferation of the cell cultures was detected bythe tetrazolium assay. The differentiation from fibroblasts to smooth muscle cells (SMCs) was deduced by measuring the expression of SMC-specific proteins (myosin and smoothelin) using immunohistochemistry and RT-PCR. Results Castration resulted in a significant decrease in circulating testosterone levels (P<0.01), but did not affect the circulating oestradiol levels (P>0.05). The prostatic stromal cells, including SMCs and fibroblasts, diminished and underwent a serial pathological change of atrophy and apoptosis after castration. The atrophic cells were filled with intracellular lipofuscin. The expression of SMC myosin declined after castration, coincident with the increase in TGF-beta mRNA level and decline in bFGF mRNA. In vitro, TGF-beta inhibited the growth of human prostatic stromal cells during exponential growth, while enhancing myosin staining and stimulating the expression of smoothelin in confluent cultured stromal cells, bFGF stimulated thegrowth of the culture and inhibited the expression of smoothelin, DHT caused a weak increase in the proliferation and expression of SMC-specific proteins (P<0.05). However, DHT and bFGF together stimulated the proliferation of stromal cells significantly more than either agent alone (P<0.01). The combination of DHT and TGF-beta greatly enhanced the expression of SMC-specific proteins (P<0.01), more strongly than either alone (P<0.01). Conclusions The whole prostate gland is an androgen-sensitive organ, with both the epithelium and stroma under the control of androgen. Androgen may direct the proliferation and differentiation of prostatic stromal cells by regulating the expression of TGF-beta and bFGF. Thus DHT, TGF-beta and bFGFmay have important roles in regulating stromal cell homeostasis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 19:56:18