Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Enhanced production of penicillin G acylase from a recombinant Escherichiacoli
Autore:
Vohra, PK; Sharma, R; Kashyap, DR; Tewari, R;
Indirizzi:
Panjab Univ, Dept Biotechnol, Chandigarh 160014, India Panjab Univ Chandigarh India 160014 Biotechnol, Chandigarh 160014, India Inst Microbial Technol, Biochem Engn & Proc Dev Ctr, Chandigarh 160036, India Inst Microbial Technol Chandigarh India 160036 Chandigarh 160036, India Panjab Univ, Dept Biotechnol, Chandigarh 160014, India Panjab Univ Chandigarh India 160014 Biotechnol, Chandigarh 160014, India
Titolo Testata:
BIOTECHNOLOGY LETTERS
fascicolo: 7, volume: 23, anno: 2001,
pagine: 531 - 535
SICI:
0141-5492(200104)23:7<531:EPOPGA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
VACCINE STRAINS; AMIDASE; CLONING; GENE;
Keywords:
Escherichia coli; penicillin G acylase gene; plasmid stability;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Tewari, R Panjab Univ, Dept Biotechnol, Chandigarh 160014, India Panjab Univ Chandigarh India 160014 , Chandigarh 160014, India
Citazione:
P.K. Vohra et al., "Enhanced production of penicillin G acylase from a recombinant Escherichiacoli", BIOTECH LET, 23(7), 2001, pp. 531-535

Abstract

Penicillin G acylase (pac) gene was cloned into a stable asd(+) vector (pYA292) and expressed in Escherichia coli. This recombinant strain produced 1000 units penicillin G acylase g(-)1 cell dry wt, which is 23-fold more than that produced by parental Escherichia coli ATCC11105. This enzyme was purified to 16 units mg(-1) protein by a novel two-step process.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 18:37:06