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Titolo:
Orthovanadate decreases leptin secretion from isolated mouse fat pads
Autore:
Suenaga, T; Hirano, K; Yoshida, A; Motoyashiki, T; Morita, T; Ueki, H;
Indirizzi:
Fukuyama Univ, Fac Pharm & Pharmaceut Sci, Dept Biochem, Fukuyama, Hiroshima 7290292, Japan Fukuyama Univ Fukuyama Hiroshima Japan 7290292 , Hiroshima 7290292, Japan
Titolo Testata:
BIOLOGICAL & PHARMACEUTICAL BULLETIN
fascicolo: 4, volume: 24, anno: 2001,
pagine: 327 - 331
SICI:
0918-6158(200104)24:4<327:ODLSFI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIPOPROTEIN-LIPASE ACTIVITY; MONOPHOSPHATE PHOSPHODIESTERASE ACTIVITY; DEPENDENT PROTEIN-KINASE; ISOLATED RAT ADIPOCYTES; WHITE ADIPOSE-TISSUE; OBESE GENE-PRODUCT; STIMULATORY RELEASE; GLYCOGEN-SYNTHASE; ADENYLATE-CYCLASE; RAPID INCREASE;
Keywords:
orthovanadate; leptin; cAMP; beta(3)-adrencrgic receptor; cAMP-dependent protein kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Ueki, H Fukuyama Univ, Fac Pharm & Pharmaceut Sci, Dept Biochem, 1 Gakuen Chou, Fukuyama, Hiroshima 7290292, Japan Fukuyama Univ 1 Gakuen Chou Fukuyama Hiroshima Japan 7290292 Japan
Citazione:
T. Suenaga et al., "Orthovanadate decreases leptin secretion from isolated mouse fat pads", BIOL PHAR B, 24(4), 2001, pp. 327-331

Abstract

When isolated mouse fat pads were incubated with orthovanadate (vanadate) or insulin for up to Ih, the leptin secretion into the medium was decreasedby vanadate and increased by insulin. Propranolol, a nonspecific antagonist of beta -adrenergic receptors, bupranorol, a specific antagonist of beta (3)-adrenergic receptor, and H-89, an inhibitor of cAMP-dependent protein kinase (PKA) all inhibited the decrease by vanadate to various extents. In contrast, no inhibition was observed with specific antagonists of beta (1)- and beta (2)-adrenergic receptors or with inhibitors of protein kinase C and Ca/calmodulin kinase. Short-term incubation of the fat pads with vanadateshowed a transient increase in the cellular cAMP content; this increase was inhibited by propranolol and bupranolol. Vanadate had no effect on the incorporation of [H-3]-leucine into proteins of the fat pads with a 4-h incubation, although insulin stimulated the incorporation, The decreasing effectof vanadate on the leptin secretion seems to he independent of the regulation of protein synthesis. These results suggest that vanadate decreases theleptin secretion through mechanisms involving the increase in cellular cAMP content via beta -adrenergic receptor, probably leading to the activationof PKA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 19:25:43