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Titolo:
Biotinylated indoles as probes for indole-binding proteins
Autore:
Dolusic, E; Kowalczyk, M; Magnus, V; Sandberg, G; Normanly, J;
Indirizzi:
Rudjer Boskovic Inst, Dept Mol Genet, HR-10002 Zagreb, Croatia Rudjer Boskovic Inst Zagreb Croatia HR-10002 t, HR-10002 Zagreb, Croatia Swedish Univ Agr Sci, Dept Forest Genet & Plant Physiol, S-90183 Umea, Sweden Swedish Univ Agr Sci Umea Sweden S-90183 t Physiol, S-90183 Umea, Sweden Univ Massachusetts, Dept Biochem & Mol Biol, Amherst, MA 01003 USA Univ Massachusetts Amherst MA USA 01003 & Mol Biol, Amherst, MA 01003 USA
Titolo Testata:
BIOCONJUGATE CHEMISTRY
fascicolo: 2, volume: 12, anno: 2001,
pagine: 152 - 162
SICI:
1043-1802(200103/04)12:2<152:BIAPFI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORANGE PERICARP; SERUM-ALBUMIN; BIOSYNTHESIS; AVIDIN; MAIZE; 2,3-DIOXYGENASE; STREPTAVIDIN; METABOLITES; MELATONIN; LYSOZYME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Magnus, V Rudjer Boskovic Inst, Dept Mol Genet, PP 180, HR-10002 Zagreb, Croatia Rudjer Boskovic Inst PP 180 Zagreb Croatia HR-10002 eb, Croatia
Citazione:
E. Dolusic et al., "Biotinylated indoles as probes for indole-binding proteins", BIOCONJ CHE, 12(2), 2001, pp. 152-162

Abstract

Biotinylated indoles were prepared for application as bifunctional probes for the detection of indole-binding proteins which participate in the life processes of humans, animals, plants, and bacteria. The indole nucleus was functionalized, at ring positions 3, 5, or 6, by attachment of a 2-aminoethyl group, which was then coupled to the carboxyl moiety of biotin, via a spacer composed of 3 or 4 concatenated beta -alanine residues. The constructsthus obtained were able to inhibit tryptophanase activity, similarly to indole in a concentration-dependent manner. They also bound strongly to lysozyme and weakly to bovine and human serum albumins, in accordance with the known affinities of these proteins for indole and 3-(2-aminoethyl)indole (tryptamine). The biotin end of the protein-bound bifunctional probes could then be detected by coupling to (strept)avidin conjugated to alkaline phosphatase or horseradish peroxidase, followed by incubation with substrates which are converted by these enzymes to intensely colored or chemiluminescent products.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 23:05:53