Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Changes induced by malathion, methylparathion and parathion on membrane lipid physicochemical properties correlate with their toxicity
Autore:
Videira, RA; Antunes-Madeira, MC; Lopes, VICF; Madeira, VMC;
Indirizzi:
Univ Coimbra, Dept Zool, Ctr Neurociencias, P-3004517 Coimbra, Portugal Univ Coimbra Coimbra Portugal P-3004517 ias, P-3004517 Coimbra, Portugal
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
fascicolo: 2, volume: 1511, anno: 2001,
pagine: 360 - 368
SICI:
0005-2736(20010402)1511:2<360:CIBMMA>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE POLARIZATION; ORGANO-PHOSPHORUS; MECHANISMS; BILAYERS; FLUIDITY; INSECTICIDES; CHOLESTEROL; LINDANE; PHOSPHATIDYLCHOLINE; DIPHENYLHEXATRIENE;
Keywords:
organophosphorus compounds; physicochemical properties of lipids; fluorescence anisotropy; differential scanning calorimetry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Madeira, VMC Univ Coimbra, Dept Zool, Ctr Neurociencias, P-3004517 Coimbra, Portugal Univ Coimbra Coimbra Portugal P-3004517 7 Coimbra, Portugal
Citazione:
R.A. Videira et al., "Changes induced by malathion, methylparathion and parathion on membrane lipid physicochemical properties correlate with their toxicity", BBA-BIOMEMB, 1511(2), 2001, pp. 360-368

Abstract

Perturbations induced by malathion, methylparathion and parathion on the physicochemical properties of dipalmitoylphosphatidylcholine (DPPC) were studied by fluorescence anisotropy of DPH and DPH-PA and by differential scanning calorimetry (DSC). Methylparathion and parathion (50 muM) increased thefluorescence anisotropy evaluated by DPH-PA and DPH. either in gel or in the fluid phase of DPPC bilayers, but mainly in the fluid phase. Parathion is more effective than methylparathion. On the other hand, malathion had almost no effect. All the three xenobiotics displaced the phase transition midpoint to lower temperature values and broadened the phase transition profile of DPPC, the effectiveness following the sequence: parathion > methylparathion >> malathion. A shifting and broadening of the phase transition was also observed by DSC. Furthermore, at methylparathion/lipid molar ratio of 1/2 and at parathion/lipid molar ratio of 1/7, the DSC thermograms displayeda shoulder in the main peak, in the low temperature side, suggesting coexistence of phases. For higher ratios, the phase transition profile becomes sharp as the control transition, but the midpoint is shifted to the previousshoulder position. Conversely to methylparathion and parathion. malathion did not promote phase separation. The overall data from fluorescence anisotropy and calorimetry indicate that the degree of effect of the insecticideson the physicochemical membrane properties correlates with toxicity to mammals. Therefore, the in vivo effects of organophosphorus compounds maybe inpart related with their ability to perturb the phospholipid bilayer structure, whose integrity is essential for normal cell function. (C) 2001 Published by Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 07:07:47