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Titolo:
Cyclosporin A reduces canalicular membrane fluidity and regulates transporter function in rats
Autore:
Yasumiba, S; Tazuma, S; Ochi, H; Chayama, K; Kajiyama, G;
Indirizzi:
Hiroshima Univ, Sch Med, Dept Internal Med 1, Minami Ku, Hiroshima 734, Japan Hiroshima Univ Hiroshima Japan 734 ed 1, Minami Ku, Hiroshima 734, Japan Onomichi Gen Hosp, Onomichi 7228508, Japan Onomichi Gen Hosp Onomichi Japan 7228508 n Hosp, Onomichi 7228508, Japan
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 354, anno: 2001,
parte:, 3
pagine: 591 - 596
SICI:
0264-6021(20010315)354:<591:CARCMF>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
BILIARY LECITHIN HYDROPHOBICITY; LIVER PLASMA-MEMBRANE; INDUCED CHOLESTASIS; BILE-ACIDS; P-GLYCOPROTEIN; HUMAN HEPATOCYTES; ORGANIC ANION; INHIBITION; SECRETION; VESICLES;
Keywords:
bile-salt export pump; biliary lipid; cholestasis; multidrug resistance 2; P-glycoprotein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Tazuma, S Hiroshima Univ, Sch Med, Dept Internal Med 1, Minami Ku, 1-2-3 Kasumi, Hiroshima 734, Japan Hiroshima Univ 1-2-3 Kasumi Hiroshima Japan 734 hima 734, Japan
Citazione:
S. Yasumiba et al., "Cyclosporin A reduces canalicular membrane fluidity and regulates transporter function in rats", BIOCHEM J, 354, 2001, pp. 591-596

Abstract

Changes of the biliary canalicular membrane lipid content can affect membrane fluidity and biliary lipid secretion in rats. The immunosuppressant cyclosporin A is known to cause intrahepatic cholestasis. This study investigated whether cyclosporin A influenced canalicular membrane fluidity by altering membrane phospholipids or transporter expression. In male Sprague-Dawley rats, a bile-duct cannula was inserted to collect bile, and sodium taurocholate was infused (100 nmol/min per 100 g) for 60 min. During steady-statetaurocholate infusion, cyclosporin A (20 mg/kg) or vehicle was injected intravenously and then bile was collected for 80 min. After killing the rats,canalicular membrane vesicles were prepared. Expression of canalicular membrane transporters was assessed by Western blotting and canalicular membrane vesicle fluidity was estimated by fluorescence polarization. Cyclosporin A reduced biliary lipid secretion along with a disproportionate reduction of lipids relative to bile acids. Cyclosporin A significantly decreased canalicular membrane fluidity along with an increase of the cholesterol/phospholipid molar ratio. Only expression of the transporter P-glycoprotein was increased by cyclosporin A. Because canalicular membrane transporter expression was largely unchanged by cyclosporin A despite a marked decrease of biliary lipid secretion, transporter activity may partly depend upon canalicular membrane fluidity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 10:08:44