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Titolo:
B lymphocyte involvement in ankylosing spondylitis: the heavy chain variable segment gene repertoire of B lymphocytes from germinal center-like foci in the synovial membrane indicates antigen selection
Autore:
Voswinkel, J; Weisgerber, K; Pfreundschuh, M; Gause, A;
Indirizzi:
Univ Klinikum Lubeck, Poliklin Rheumatol, Dept Rheumatol, D-23538 Lubeck, Germany Univ Klinikum Lubeck Lubeck Germany D-23538 tol, D-23538 Lubeck, Germany Univ Saarlandes Kliniken, Homburg, Germany Univ Saarlandes Kliniken Homburg Germany des Kliniken, Homburg, Germany
Titolo Testata:
ARTHRITIS RESEARCH
fascicolo: 3, volume: 3, anno: 2001,
pagine: 189 - 195
SICI:
1465-9913(2001)3:3<189:BLIIAS>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
RHEUMATOID-ARTHRITIS RA; VH GENE; NUCLEOTIDE-SEQUENCE; IMMUNE-RESPONSE; CELLS; ANTIBODIES; BLOOD; DISEASE; REVEALS; SINGLE;
Keywords:
ankylosing spondylitis; B lymphocyte immunology; heavy chain genes; immunoglobulins; somatic mutations;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Voswinkel, J Univ Klinikum Lubeck, Poliklin Rheumatol, Dept Rheumatol, Ratzeburger Allee 160, D-23538 Lubeck, Germany Univ Klinikum Lubeck Ratzeburger Allee 160 Lubeck Germany D-23538
Citazione:
J. Voswinkel et al., "B lymphocyte involvement in ankylosing spondylitis: the heavy chain variable segment gene repertoire of B lymphocytes from germinal center-like foci in the synovial membrane indicates antigen selection", ARTHRITIS R, 3(3), 2001, pp. 189-195

Abstract

The synovial membrane (SM) of affected joints in ankylosing spondylitis (AS) is infiltrated by germinal center-like aggregates (foci) of lymphocytes similar to rheumatoid arthritis (RA). We characterized the rearranged heavychain variable segment (VH) genes in the SM for gene usage and the mutational pattern to elucidate the B lymphocyte involvement in AS. Cryosections from an AS-derived SM were stained for B and T lymphocytes. Bcells were isolated from different areas of a focus. The rearranged VH genes were amplified by semi-nested polymerase chain reaction (PCR) using oligonucleotides specific for the six different VH families and heavy chain joining segments (JHs). PCR products were cloned and sequenced. Fifty-nine of 70 different heavy chain gene rearrangements were potentially functional. Most of the rearranged genes were mutated (range, 1-15%). Thirty of 70 products had a mutational pattern typical for antigen selection. Most of the rearranged VH genes belonged to the VH3 family (54%), consistent with data from healthy donors and patients with RA, while VH4 genes, in contrast to RA, were identified less frequently (10%) and VH5 genes were over-represented (11%). In contrast to RA, neither VH6 genes nor the autoimmunity-prone VH4-34 were seen, whereas another autoimmunity-prone gene, V3-23,was predominantly used (11%). One VH1-derived and one VH3-derived B cell clone were expanded. CDR3 were shorter and more variable in length than in RA. Comparable with RA and reactive arthritis, there is a biased repertoire ofrepresented genes is different and less clonal expansion was observed.

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Documento generato il 26/09/20 alle ore 05:34:35