Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Policosanol modulates HMG-CoA reductase activity in cultured fibroblasts
Autore:
Menendez, R; Amor, AM; Rodeiro, I; Gonzalez, RM; Gonzalez, PC; Alfonso, JL; Mas, R;
Indirizzi:
Ctr Nacl Invest Cient, Ctr Prod Nat, Lab Bioquim, Havana, Cuba Ctr Nacl Invest Cient Havana Cuba r Prod Nat, Lab Bioquim, Havana, Cuba
Titolo Testata:
ARCHIVES OF MEDICAL RESEARCH
fascicolo: 1, volume: 32, anno: 2001,
pagine: 8 - 12
SICI:
0188-4409(200101/02)32:1<8:PMHRAI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
COENZYME-A REDUCTASE; LOW-DENSITY-LIPOPROTEIN; LINE HEP G2; II HYPERCHOLESTEROLEMIA; LIPID PROFILE; EFFICACY; TOLERABILITY; DEGRADATION; INHIBITORS; SAFETY;
Keywords:
policosanol; cholesterol biosynthesis; HMG-CoA reductase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Menendez, R Ctr Nacl Invest Cient, Ctr Prod Nat, Lab Bioquim, Ave 25 & 158,Cabanacan,Playa,POB 6880, Havana, Cuba Ctr Nacl Invest Cient Ave 25 & 158,Cabanacan,Playa,POB 6880 Havana Cuba
Citazione:
R. Menendez et al., "Policosanol modulates HMG-CoA reductase activity in cultured fibroblasts", ARCH MED R, 32(1), 2001, pp. 8-12

Abstract

Background. Cholesterol biosynthesis is strictly controlled by 3-hydroxy-3-methylglutaryl Coenzyme A (HMG-CoA) reductase. Methods. Transfer of cultured fibroblasts to a lipid-depleted medium (LDM) up-regulates the enzyme levels. This, in turn, is followed by an accelerated biosynthesis of cholesterol,Results. Exposure of Vero fibroblasts to LDM and policosanol (0.5-50 mug/mL), a new cholesterol-lowering drug purified from sugarcane (Saccharum officinarum L.) wax, decreased in a dose-dependent manner cholesterol biosynthesis from [C-14]-acetate and H-3-water, but not from [C-14]-mevalonate. Conclusions. This suggests an effect on HMG-CoA reductase, the rate-controlling enzyme in cholesterol biosynthesis. When enzyme activity was measuredin the presence of various concentrations of policosanol (0.5-50 mug/mL), reductase was not suppressed. Therefore, there was no evidence for a competitive or noncompetitive inhibition of enzyme activity. However, after treatment of intact cells with policosanol (50 mug/mL) in the presence of LDM, asuppressive effect on enzyme activity was observed, suggesting a modulatory effect of policosanol on reductase activity. The previous inhibition of enzyme up-regulation by policosanol suggests to date a depression of de novosynthesis of HMG-CoA reductase and/or stimulation of its degradation. However, the exact mechanism by which policosanol inhibits the activity of HMG-CoA reductase still remains unclear, Further studies are needed to clarify the precise mechanism of its inhibitory action on cholesterol biosynthesis. (C) 2001 IMSS. Published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:20:29