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Titolo:
Induction of apoptosis by the marine sponge (Mycale) metabolites, mycalamide A and pateamine
Autore:
Hood, KA; West, LM; Northcote, PT; Berridge, MV; Miller, JH;
Indirizzi:
Victoria Univ Wellington, Sch Biol Sci, Wellington 6001, New Zealand Victoria Univ Wellington Wellington New Zealand 6001 n 6001, New Zealand Victoria Univ Wellington, Sch Chem & Phys Sci, Wellington 6001, New Zealand Victoria Univ Wellington Wellington New Zealand 6001 n 6001, New Zealand Malaghan Inst Med Res, Wellington S, New Zealand Malaghan Inst Med Res Wellington S New Zealand ellington S, New Zealand
Titolo Testata:
APOPTOSIS
fascicolo: 3, volume: 6, anno: 2001,
pagine: 207 - 219
SICI:
1360-8185(200106)6:3<207:IOABTM>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEW-ZEALAND SPONGE; PROTEIN-KINASE-C; CELL-DEATH; SIGNALING PATHWAY; EPITHELIAL-CELLS; ANTITUMOR AGENTS; RAS; ACTIVATION; INHIBITORS; ABL;
Keywords:
apoptosis; mycalamide; pateamine; 32D cells; ras; bcr/abl;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Miller, JH Victoria Univ Wellington, Sch Biol Sci, POB 600, Wellington 6001, New Zealand Victoria Univ Wellington POB 600 Wellington New Zealand 6001 d
Citazione:
K.A. Hood et al., "Induction of apoptosis by the marine sponge (Mycale) metabolites, mycalamide A and pateamine", APOPTOSIS, 6(3), 2001, pp. 207-219

Abstract

The marine sponge metabolites mycalamide A (myca-lamide) and pateamine areextremely cytotoxic. While mycalamide has been shown to inhibit protein synthesis, the mechanism by which these compounds induce cell death is unknown. Using DNA laddering, Annexin-V staining, and morphological analysis, we demonstrate that both metabolites induce apoptosis in several different cell lines. Furthermore, both mycalamide and pateamine were more potent inducers of apoptosis in the 32D myeloid cell line after transformation with either the ras or bcr-abl oncogenes. This increased sensitivity was also observed in response to the protein synthesis inhibitors cycloheximide and puromycin, and cytosine-beta -D-arabinofurano-side (Ara-C), an inducer of DNA damage. We propose, therefore, that in 32D cells where Ras signalling has beenaltered either by constitutive expression of oncogenic ras or by Bcr/abl-mediated perturbation of upstream signalling events, increased susceptibility to apoptosis by a range of stimuli is conferred.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:18:37