Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Molecular detection of bacterial and streptomycete chitinases in the environment
Autore:
Williamson, N; Brian, P; Wellington, EMH;
Indirizzi:
Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England Univ Warwick Coventry W Midlands England CV4 7AL 7AL, W Midlands, England TerraGen Discovery Inc, Vancouver, BC, Canada TerraGen Discovery Inc Vancouver BC Canada ry Inc, Vancouver, BC, Canada
Titolo Testata:
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
fascicolo: 3-4, volume: 78, anno: 2000,
pagine: 315 - 321
SICI:
0003-6072(200012)78:3-4<315:MDOBAS>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEQUENCES; GENE; COMMUNITY; DNA;
Keywords:
actinomycete; chitinase; DGGE; diversity; DNA; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Wellington, EMH Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands,England Univ Warwick Coventry W Midlands England CV4 7AL England
Citazione:
N. Williamson et al., "Molecular detection of bacterial and streptomycete chitinases in the environment", ANTON LEEUW, 78(3-4), 2000, pp. 315-321

Abstract

Sets of PCR primers were designed to amplify bacterial chitinases at different levels of specificity. The bacterial chitinase group primers were successful in targeting enzymes classified within the group A glycosyl hydrolases of family 18. The widespread occurrence of group A bacterial chitinases in actinomycetes was demonstrated. Streptomycete chitinase specific primerswere designed and a collection of type strains of species changed in the genes Streptomyces were screened and shown to have at least one and usually multiple chitinase genes. The presence of the gene for the chitin binding protein was also demonstrated within the streptomycete type strains. These data indicate that streptomycetes are well equipped to degrade chitin. The detection of group A chitinases in total community DNA is described and a sandy soil shown to contain more than 10 different genes using DGGE to indicate genetic diversity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 06:22:13