Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Digitonin enhances the anti-tumor effect of cisplatin against methylcholanthrene-induced rat sarcoma cells in vitro
Autore:
Tanaka, T; Kaneda, Y; Li, TS; Matsuoka, T; Zempo, N; Esato, K;
Indirizzi:
Yamaguchi Univ, Sch Med, Dept Surg 1, Ube, Yamaguchi 7558505, Japan Yamaguchi Univ Ube Yamaguchi Japan 7558505 Ube, Yamaguchi 7558505, Japan
Titolo Testata:
ANTICANCER RESEARCH
fascicolo: 1A, volume: 21, anno: 2001,
pagine: 313 - 315
SICI:
0250-7005(200101/02)21:1A<313:DETAEO>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED LUNG PERFUSION; PLASMA-MEMBRANE; PERMEABILIZATION;
Keywords:
digitonin cisplatin; anti-tunlor effectf; permeabilization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Tanaka, T Yamaguchi Univ, Sch Med, Dept Surg 1, 1-1-1 Minami Kogushi, Ube,Yamaguchi7558505, Japan Yamaguchi Univ 1-1-1 Minami Kogushi Ube Yamaguchi Japan 7558505
Citazione:
T. Tanaka et al., "Digitonin enhances the anti-tumor effect of cisplatin against methylcholanthrene-induced rat sarcoma cells in vitro", ANTICANC R, 21(1A), 2001, pp. 313-315

Abstract

Background: This study was designed to evaluate cellular uptake and cytotoxicity of cisplatin in methylcholanthrene (MCA)-induced rat sarcoma cells M,hen used in combination with a detergent, digitonin. Materials and Methods: In the cellular intake study, after MCA sarcoma cells (10(7)) were treated with cisplatin alone (50 mug/ml) and with cisplatin (50 mug/ml) in combination with digitonin at 20 muM and 50 muM, the cells were washed twice withPBS and the platinum levels were measured by flameless atomic spectrometry. For the anti-tumor. effect MCA sarcoma cells (10(3)) were seeded in cell culture dishes and loaded for 10 minutes with PBS, digitonin (5 muM), cisplatin (5 mug/ml) or a combination of cisplatin (5 mug/ml) and digitonin (5 muM). The cells were then washed and incubated for 72 hours. Bromodeoxyrudine uptake was measured with an ELISA system for determining viable cells counts. Results: Cell platinum levels were significantly elevated in proportion to the increase of digitonin (p < 0.0001). The number of viable cells wassignificantly decreased with, the combined cisplatin (5 mug/ml) - digitonin (5 muM) treatment (p <0.0001 Conclusion: Digitonin enhances tile antitumor effect of cisplatin against methylcholanthrene-induced Int sarcoma in vitro.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 13:10:56