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Titolo:
Sequential gene expression of P-glycoprotein (P-gp), multidrug resistance-associated protein (MRP) and lung resistance protein: functional activity of P-gp and MRP present in the doxorubicin-resistant human K562 cell lines
Autore:
Grandjean, F; Bremaud, L; Verdier, M; Robert, J; Ratinaud, MH;
Indirizzi:
Fac Med Limoges, Grp Physiol Mol Mitochondriale & Immunitaire, CNRS, UMR 6101, F-87025 Limoges, France Fac Med Limoges Limoges France F-87025 UMR 6101, F-87025 Limoges, France Fac Sci, Inst Biotechnol, F-87060 Limoges, France Fac Sci Limoges FranceF-87060 Inst Biotechnol, F-87060 Limoges, France Inst Bergonie, F-33076 Bordeaux, France Inst Bergonie Bordeaux France F-33076 Bergonie, F-33076 Bordeaux, France
Titolo Testata:
ANTI-CANCER DRUGS
fascicolo: 3, volume: 12, anno: 2001,
pagine: 247 - 258
SICI:
0959-4973(200103)12:3<247:SGEOP(>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
DRUG-RESISTANCE; MONOCLONAL-ANTIBODY; TUMOR-CELLS; CANCER; OVEREXPRESSION; TRANSPORTER; ADRIAMYCIN; VERAPAMIL; REVERSAL; RNA;
Keywords:
doxorubicin; lung resistance protein; multidrug resistance-associated protein; P-glycoprotein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Ratinaud, MH Fac Med Limoges, Grp Physiol Mol Mitochondriale & Immunitaire, CNRS, UMR 6101, 2 Rue Dr Marcland, F-87025 Limoges, France Fac Med Limoges 2 Rue Dr Marcland Limoges France F-87025 nce
Citazione:
F. Grandjean et al., "Sequential gene expression of P-glycoprotein (P-gp), multidrug resistance-associated protein (MRP) and lung resistance protein: functional activity of P-gp and MRP present in the doxorubicin-resistant human K562 cell lines", ANTI-CANC D, 12(3), 2001, pp. 247-258

Abstract

Previous studies have reported that P-glycoprotein (P-gp), a transmembraneefflux pump involved in multidrug resistance (MDR), was overexpressed in the doxorubicin (Dox)-resistant human erythroleukemia cell line K562. Nevertheless, several results suggested that P-gp was not the only mechanism involved in these resistant cells. Sequential co-expression of other Mon-associated proteins was sometimes reported, as MDR-associated protein (MRP) and lung resistance protein (LRP), in different MDR cell lines. Thus, mRNA expression and stability of P-gp, MRP and LRP were analyzed, while their corresponding protein levels were quantified in correlation with functional assay,in the K562 cell line and two Dox-resistant variants (K562/R). Their P-gp content was in accordance with their degree of resistance, but not as much in the level of mRNA expression, suggesting a posttranscriptional regulation. On the other hand, MRP could play a minor role in Mon because of an unchanged expression in K562/R sublines. A surprising progressive disappearanceof LRP in both resistant cells suggested that the original mechanism of drug redistribution may be operative, involving a negative role for LRP. [(C)2001 Lippincott Williams & Wilkins].

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 09:12:02