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Titolo:
Maturation of carbonic anhydrase IV expression in rabbit kidney
Autore:
Winkler, CA; Kittelberger, AM; Watkins, RH; Maniscalco, WM; Schwartz, GJ;
Indirizzi:
Univ Rochester, Sch Med, Dept Pediat, Div Pediat Nephrol, Rochester, NY 14642 USA Univ Rochester Rochester NY USA 14642 at Nephrol, Rochester, NY 14642 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 5, volume: 280, anno: 2001,
pagine: F895 - F903
SICI:
0363-6127(200105)280:5<F895:MOCAIE>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONVOLUTED TUBULE ACIDIFICATION; MEDULLARY COLLECTING DUCT; PROXIMAL TUBULE; POSTNATAL MATURATION; DEVELOPMENTAL-CHANGES; ANTIPORTER ACTIVITY; INTERCALATED CELLS; SOLUTE TRANSPORT; RAT-KIDNEY; LOCALIZATION;
Keywords:
kidney development; in situ hybridization; immunohistochemistry; Northern blot; medullary collecting duct; proximal tubule; intercalated cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Schwartz, GJ Univ Rochester, Sch Med, Dept Pediat, Div Pediat Nephrol, Box777,601 Elmwood Ave, Rochester, NY 14642 USA Univ Rochester Box 777,601 Elmwood Ave Rochester NY USA 14642
Citazione:
C.A. Winkler et al., "Maturation of carbonic anhydrase IV expression in rabbit kidney", AM J P-REN, 280(5), 2001, pp. F895-F903

Abstract

Carbonic anhydrase (CA) IV facilitates renal acidification by catalyzing the dehydration of luminal H2CO3. CA IV is expressed in proximal tubules, medullary collecting ducts, and A-intercalated cells of the mature rabbit kidney (Schwartz GJ, Kittelberger AM, Barnhart DA, and Vijayakumar S. Am J Physiol 278: F894-F904, 2000). In view of the maturation of HCO3- transport inthe proximal tubule and collecting duct, the ontogeny of CA IV expression was examined. During the first 2 wk, CA IV mRNA was expressed in maturing cortex and medulla at similar to 20% of adult levels. The maturational increase was gradual in cortex over 3-5 wk of age but surged in the medulla, so that mRNA levels appeared higher than those in the adult medulla. In situ hybridization showed very little CA IV mRNA at 5 days, with increases in deep cortex and medullary collecting ducts by 21 days. Expression of CA IV protein in the cortex and medulla was minimal at 3 days of age but then apparent in the juxtamedullary region, A-intercalated cells and medullary collecting ducts by 18 days; there was little labeling of the proximal straight tubules of the medullary rays. Thus CA IV expression may be regulated to accommodate the maturational increase in HCO3- absorption in the proximal tubule. In the medullary collecting duct, there is a more robust maturation of CA IV mRNA and protein, commensurate with the high rate of HCO3- absorption in the neonatal segment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 15:18:14