Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
SFKs, Ras, and the classic MAPK pathway couple muscarinic receptor activation to increased Na-HCO3 cotransport activity in renal epithelial cells
Autore:
Robey, RB; Ruiz, OS; Baniqued, J; Mahmud, D; Espiritu, DJD; Bernardo, AA; Arruda, JAL;
Indirizzi:
Univ Illinois, Coll Med, Dept Med, Nephrol Sect, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 d, Nephrol Sect, Chicago, IL 60612 USA Univ Illinois, Coll Med, Dept Physiol & Biophys, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 ysiol & Biophys, Chicago, IL 60612 USA Univ Illinois, Coll Med, Dept Pathol, Chicago, IL 60612 USA Univ IllinoisChicago IL USA 60612 ed, Dept Pathol, Chicago, IL 60612 USA Vet Affairs Chicago Hlth Care Syst, W Side Div, Chicago, IL 60612 USA Vet Affairs Chicago Hlth Care Syst Chicago IL USA 60612 ago, IL 60612 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
fascicolo: 5, volume: 280, anno: 2001,
pagine: F844 - F850
SICI:
0363-6127(200105)280:5<F844:SRATCM>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROXIMAL CONVOLUTED TUBULE; HEXOKINASE-ACTIVITY; KINASE ACTIVATION; TYROSINE KINASES; GENE-TRANSFER; SRC KINASE; PROTEIN; OVEREXPRESSION; STIMULATION; MODULATION;
Keywords:
sodium-bicarbonate cotransport; proximal tubule; epithelial cell; mitogen-activated protein kinase; Src family kinases; carboxyterminal Src kinase; Ras; intracellular pH;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Arruda, JAL Univ Illinois, Coll Med, Dept Med, Nephrol Sect, 820 S Wood St,Rm 418W CSN,M-C 793, Chicago, IL 60612 USA Univ Illinois 820 S Wood St,Rm 418W CSN,M-C 793 Chicago IL USA 60612
Citazione:
R.B. Robey et al., "SFKs, Ras, and the classic MAPK pathway couple muscarinic receptor activation to increased Na-HCO3 cotransport activity in renal epithelial cells", AM J P-REN, 280(5), 2001, pp. F844-F850

Abstract

Cholinergic agents are known to affect the epithelial transport of H2O andelectrolytes in the kidney. In proximal tubule cells, cholinergic agonistsincrease basolateral Na-HCO3 cotransport activity via M-1 muscarinic receptor activation. The signaling intermediates that couple these G protein-coupled receptors to cotransporter activation, however, are not well defined. We therefore sought to identify distal effectors of muscarinic receptor activation that contribute to increased NBC activity in cultured proximal tubule cells. As demonstrated previously for acute CO2-regulated cotransport activity, we found that inhibitors of Src family kinases (SFKs) or the classic mitogen-activated protein kinase (MAPK) pathway prevented the stimulationof NBC activity by carbachol. The ability of carbachol to activate Src, aswell as the proximal (Raf) and distal [extracellular signal-regulated kinases 1 and 2 (ERK1/2)] elements of the classic MAPK module, was compatible with these findings. Cholinergic stimulation of ERK1/2 activity was also completely prevented by overexpression of a dominant negative mutant of Ras (N17-Ras). Taken together, these findings suggest a requirement for the sequential activation of SFKs, Ras, and the classic MAPK pathway [Raf-->MAPK/ERKkinase (MEK) --> ERK]. These findings provide important insights into the molecular mechanisms underlying cholinergic regulation of NBC activity in renal epithelial cells. They also suggest a specific mechanism whereby cholinergic stimulation of the kidney can contribute to pH homeostasis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:41:52