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Titolo:
Deformation-induced lipid trafficking in alveolar epithelial cells
Autore:
Vlahakis, NE; Schroeder, MA; Pagano, RE; Hubmayr, RD;
Indirizzi:
Mayo Clin & Mayo Fdn, Dept Internal Med, Div Pulm & Crit Care Med, Thorac Dis Res Unit, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 Unit, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Dept Biochem & Mol Biol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 Biol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA MayoClin & Mayo Fdn Rochester MN USA 55905 phys, Rochester, MN 55905 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 5, volume: 280, anno: 2001,
pagine: L938 - L946
SICI:
1040-0605(200105)280:5<L938:DLTIAE>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
END-EXPIRATORY PRESSURE; PLASMA-MEMBRANE; SURFACE-AREA; SPECTRAL PROPERTIES; LUNG INJURY; RAT LUNG; VOLUME; TENSION; EXOCYTOSIS; EXPRESSION;
Keywords:
mechanical ventilation; BODIPY lipids; plasma membrane; lung injury; deforming stress;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Vlahakis, NE Mayo Clin & Mayo Fdn, Dept Internal Med, Div Pulm & Crit CareMed, Thorac Dis Res Unit, Rm 4-411 Alfred Bldg,20 1st St SW, Rochester, MN55905 USA Mayo Clin & Mayo Fdn Rm 4-411 Alfred Bldg,20 1st St SW RochesterMN USA 55905
Citazione:
N.E. Vlahakis et al., "Deformation-induced lipid trafficking in alveolar epithelial cells", AM J P-LUNG, 280(5), 2001, pp. L938-L946

Abstract

Mechanical ventilation with a high tidal volume results in lung injury that is characterized by blebbing and breaks both between and through alveolarepithelial cells. We developed an in vitro model to simulate ventilator-induced deformation of the alveolar basement membrane and to investigate, in a direct manner, epithelial cell responses to deforming forces. Taking advantage of the novel fluorescent properties of BODIPY lipids and the fluorescent dye FM1-43, we have shown that mechanical deformation of alveolar epithelial cells results in lipid transport to the plasma membrane. Deformation-induced lipid trafficking (DILT) was a vesicular process, rapid in onset, and was associated with a large increase in cell surface area. DILT could bedemonstrated in all cells; however, only a small percentage of cells developed plasma membrane breaks that were reversible and nonlethal. Therefore, DILT was not only involved in site-directed wound repair but might also have served as a cytoprotective mechanism against plasma membrane stress failure. This study suggests that DILT is a regulatory mechanism for membrane trafficking in alveolar epithelia and provides a novel biological framework within which to consider alveolar deformation injury and repair.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 09:58:47